Thidiazuron对解除羊草种子休眠和植株再生的影响

邹吉祥; 金华; 姜国斌; 黄磊

doi:10.11829/j.issn.1001-0629.2015-0189

Thidiazuron对解除羊草种子休眠和植株再生的影响

大连民族大学,辽宁大连 116600

基金项目:

科技部国际合作计划(2010DFA91930)

国家自然科学基金(31100452)

摘要: 研究了不同浓度植物生长调节剂噻苯隆(Thidiazuron,TDZ)对解除羊草(Leymus chinensis)种子休眠和植株再生的影响,并对愈伤组织进行超微结构观察。结果表明,0.02 mg·L-1 TDZ低温浸种对解除羊草种子休眠和诱导胚性愈伤组织的效果最佳,发芽率和出愈率分别为11.0%和15.5%;羊草愈伤组织分化最佳培养基配方为MS+TDZ 0.5 mg·L-1+CH 2.0 g·L-1,分化率、绿芽分化率分别为56.7%和423.3%;TDZ低温浸泡处理的羊草种子诱导出的胚性愈伤组织较非胚性愈伤组织细胞质体中内含物丰富,更易于体胚形成;丛生芽经过1/2MS生根培养基的3次继代,炼苗移栽后成活率达100%。

关键词:

羊草 /
TDZ /
休眠 /
再生 /
超微观察

English

Influence of Thidiazuron on dormancy release of Leymus chinensis seed and plant regeneration

College of Environment and Resources, Dalian Nationalities University , Dalian 116600, China

Received Date: 2015-04-02
Publish Date: 2015-10-19

Abstract: In the experiment, dormancy release of Leymus chinensis seed and plant regeneration is studied using plant growth regulator TDZ with different concentrations, and ultramicro observations are conducted on callus. The results showed that 0.02 mg·L-1 TDZ cold soaking has the best effect on dormancy release of L. chinensis seed and embryonic callus induction. The germination rate and the healing rate are 11% and 15.5%, respectively, the best media component formula for L. chinensis callus differentiation is MS+TDZ 0.5 mg·L-1+CH 2.0 g·L-1, the differentiation rate and green shoot differentiation rate are 56.7% and 423.3%, respectively. Otherwise, transmission electron microscope (TEM) observations found that the embryonic callus induced by TDZ cold soaking, L. chinensis seeds contain more rich contents and are more conductive to body embryo formation than that of non-embryonic callus cell plasmid. After acclimatization and transplanting, the survival rate of the multiple shoots through 3 times of subculture with 1/2 MS rooting media is 100%.

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参考文献(1)

[1]

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Thidiazuron对解除羊草种子休眠和植株再生的影响

English

Influence of Thidiazuron on dormancy release of Leymus chinensis seed and plant regeneration

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