马蔺重金属ATP酶基因<em> HMA2</em>的RNAi载体构建及其遗传转化

马蔺重金属ATP酶基因 HMA2的RNAi载体构建及其遗传转化

张亚楠^1,², 蔺银鼎¹, 孟林², 毛培春², 田小霞², 郭强²

Construction of RNAi expression vector of heavy metal ATPases gene HMA2 and genetic transformation in Iris lactea

Zhang Ya-nan^1,², Lin Yin-ding¹, Meng Li², Mao Pei-chun², Tian Xiao-xia², Guo Qiang²

马蔺 HMA2 -RNAi载体的双酶切验证注:M₂,Trans2K Plus Ⅱ DNA Marker;泳道1,XhoⅠ/KpnⅠ酶切产物;泳道2,KpnⅠ/ClaⅠ酶切产物;泳道3,XbaⅠ/ClaⅠ酶切产物;泳道4,XhoⅠ/ClaⅠ酶切产物;泳道5,SacⅠ/SpeⅠ酶切产物。