Establishment of singlestrandconformation polymorphism (SSCP) for microbial community Structure in goose intestine

PCRSSCP is one of rapid, simple and sensitive analytic technology to examine genome adopting DNA single strand conformation. However, many factors influence the results of SSCP analysis. In order to study microbial community in goose intestine, various controlled conditions such as the gelconcentration, the concentration of crosslinker, the dilution of the PCR products, the electrophoresis temperature, voltage, the presence of glycerol, urea and Lambda exonuclease in gels during electrophoresis were studied. Results showed that while the gelconcentration is 12% (acrylamidebisacrylamide 39∶1), no glycerol, and the voltage is 120 V, with constant temperature during electrophoresis were the best SSCP conditions.