Cloning and sequencing of GAPDH gene from Vicia sativa

To provide molecular basis of highaltitude adaption, a partial sequence of glyceraldehyde3phosphate dehydrogenase (GAPDH) gene was isolated from common vetch (Vicia sativa). In this study, a pair of primers was designed according to homologous GAPDH genes among closely related plants, and then the reverse transcription PCR (RTPCR) was performed. A nucleotide sequence of 1 141 bp with coding 381 animo acides was identified. Homological analysis showed that the similarity between this sequence and GAPDH gene sequences of other organisms was over 83% in nucleoride sequence level and over 95% in animo acid level. Therefore, the sequence was considered to be V. sativa GAPDH gene. It was named VsGAPDH, and was submited to GenBank with accession number HM117006.