Tissue culture and rapid propagation of Chrysanthemum grandiflorum cv.White Snow

The stems with axillary bud of Chrysanthemum grandiflorum cv.White Snow were used as explants and the sterile material was obtained from disinfecting．The tissue culture and rapid propagation technique were studied by using MS as basal medium with different concentrations of hormones．The results showed that the best medium for adventitious bud induction from leaves was MS+1.0 mgL-16BA+0.7 mgL-1NAA，which had the highest induction rate (100％)and the highest differentiation rate (63.3％)．The best medium for proliferation of stems segment was MS+0.3 mgL-16BA+0.2 mgL-1NAA，which had the average multiplication coefficient (6.73)．The subculture medium was MS．The best medium for taking root was 1/2MS+0.5 mgL-1NAA，on which the rooting rate was 100％and the average rooting coefficient was 15.8．The transferred plants had a survival rate of 100％ and grew well．