Isolation and identification of lactic acid bacteria from previously fermented juice of Italian ryegrass

In order to study lactic acid bacteria species which plays a major role in the fermentation process of Italian ryegrass(Lolium multiflorum) silage, 6 lactic acid bacteria strains(L-1, L-3, L-4, L-5, L-8 and L-9) were isolated from previously fermented juice of Changjiang No.2 Italian ryegrass. The results of traditional biochemical identification, experiment of acid-production rate, acid tolerance test, salt tolerance test, and the 16S rRNA sequence analysis indicated that L-1, L-3, L-4, L-5, L-8 and L-8 are Lactobacillus plantarum, Lactococcus lactis, Lactococcus garvieae, Leuconostoc lactis, Weissella confuse and Weissella cibaria, respectively, and all of them have good acid productivity, strong acid and salt tolerance. Further studies on using these strains as feed additives can be made.