Welcome Pratacultural Science,Today is 2025-5-2 Friday!
The effect of xylose on the callus growth of perennial ryegrass[J]. Pratacultural Science, 2011, 5(5): 758-762.
Citation: The effect of xylose on the callus growth of perennial ryegrass[J]. Pratacultural Science, 2011, 5(5): 758-762.

The effect of xylose on the callus growth of perennial ryegrass

More Information
  • Published Date: May 14, 2011
  • Xylose as a selective agent is considered to be biosafe for plant genetic transformation. For investigating the possibility of xylose isomerase gene as a selective marker in genetic transformation of perennial ryegrass (Lolium perenne), the effect of xylose on the growth of calli which derived from mature embryos of perennial ryegrass was detected. Cultured on subcultivation medium (SM) with different proportions of sucrose, xylose and sucrose+xylose respectively,the calli were analyzed and their weight gain were determined after 5 week culture. The results of this study showed that the weight gain of calli increased with the enhancing of sugar content from 0 to 30 g/L. Calli grew much better on the media with sucrose than with xylose at the same concentration. At 30 g/L total amount of sugar in the mixed media, the weight gain of calli declined when the proportion of sucrose decreased in the meanwhile xylose increased. These results presented that xylose restricted the callus growth evidently. Moreover, on the media of SM+5 g/L sucrose+25 g/L xylose or SM+30 g/L xylose, the biomass increment was lower than others. It was suggested that xylose could be employed as a positive selective agent in perennial ryegrass genetic transformation at the selection pressure of 25 g/L xylose+5 g/L sucrose or 30 g/L xylose.
  • Related Articles

    [1]ZHANG Jinpeng, GAO Shikong, YU Qiao, ZHAO Yifan, WANG Zongsen, GAO Junjun, HAO Xiaojun, HU Tianming, FU Juanjuan. Effects of exogenous Putrescine on plant growth and photosynthesis of perennial ryegrass under drought stress[J]. Pratacultural Science. DOI: 10.11829/j.issn.1001-0629.2024-0045
    [2]CHEN Yanqi, LIU Jiayuan, LIU Wenke, WANG Qi. Effects of red and blue light intensities on the growth, yield, and quality of perennial ryegrass[J]. Pratacultural Science, 2023, 40(2): 502-510. DOI: 10.11829/j.issn.1001-0629.2022-0487
    [3]LIU Peng, CUI Tongtong, SUN Pengyue, ZHOU Yu, ZHANG Zhiwei, YU Xinrong, HU Tianming, FU Juanjuan. Effects of Arthrobacter psychrolactophilus B7 on the growth and antioxidant defense system in perennial ryegrass under shade stress[J]. Pratacultural Science, 2021, 38(1): 81-88. DOI: 10.11829/j.issn.1001-0629.2020-0140
    [4]SU Yanning, WANG Ruijia, CAI Jiabang, TANG Mingyu, LIAO Zongchao, YANG Xinying, ZHANG Xinquan, NIE Gang. Effects of jasmonic acid pretreatment on perennial ryegrass seed germination under heat stress[J]. Pratacultural Science, 2020, 37(4): 658-668. DOI: 10.11829/j.issn.1001-0629.2019-0443
    [5]Yan Fan, Dan-dan Min, Zheng-gang Guo, Bao-chang Feng, Xiao-wen Hu. Comparison of the salt tolerance of perennial ryegrass during seed germination and seedling growth[J]. Pratacultural Science, 2017, 11(4): 724-734. DOI: 10.11829/j.issn.1001-0629.2016-0228
    [6]Wei-hu Lin, Jian-jun Wang, Hui-qiang Li, Guang-ming Zhang, Pei Tian. Effects of endophytic fungi on perennial ryegrass physiological characteristics under different growth conditions[J]. Pratacultural Science, 2016, 10(8): 1574-1582. DOI: 10.11829/j.issn.1001-0629.2016-0131
    [7]Hui-qiang Li, Jian-jun Wang, Guang-ming Zhang, Wei-hu Lin, Pei Tian. Effects of fungal endophytes on the growth of perennial ryegrass under drought condition[J]. Pratacultural Science, 2016, 10(4): 599-607. DOI: 10.11829/j.issn.1001-0629.2016-0029
    [8]Yi-kai LIU, Ming-zhu ZHENG, Li MA, Tie-jun LIU, You-jun YANG, Jin-rong LIU. Effects of regulated deficit irrigation on photosynthetic characteristics in Perennial Ryegrass and the determination for irrigation threshold[J]. Pratacultural Science, 2015, 9(4): 570. DOI: 10.11829/j.issn.1001-0629.2014-0196
    [9]Hui-fang LI, Qing-hua YUAN, Gui-qin ZHAO. Effects of cadmium stress on seedling growth of perennial ryegrass germplasm[J]. Pratacultural Science, 2014, 8(5): 898-904. DOI: 10.11829/j.issn.1001-0629.2013-0257
    [10]Xinyun CAI, Qiuyu SHAO, Xinxin ZHANG, Ruixin WU. Effects of plant growth regulators on growth characteristics of Lolium perenne[J]. Pratacultural Science, 2013, 7(7): 1014-1018.

Catalog

    Article views (2108) PDF downloads (1249) Cited by()

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return