Genetic diversity analysis of 20 Dichondra repens germplasm by RAPD and SRAP

Genetic diversity of 20 Dichondra repens wild germplasm collected from different location in southwest China, compared with an imported American variety (Common), were analyzed by RAPD and SRAP markers. The results showed that 20 RAPD primers produced 189 bands, including 130 polymorphic bands; the average percentage of polymorphic bands was 68.78%. 22 pairs of SRAP primers produced 242 bands, including 175 polymorphic bands; the average percentage of polymorphic bands was 72.31%. The 21 germplasm could be clustered into either two groups based on RAPD markers as well as SRAP markers. Moreover, the tested germplasm with same origin or similar morphology frequently clustered into the same group and showed a nice geographical distribution and relationships. There was significant positive correlation between RAPD and SRAP (r0.01=0.910), which indicated that the two methods were high consistant and reliable for detecting genetic diversity and genetic relationships in D.repens germplasms, though some germplasm were clustered into different groups between the two markers.