Enzymolysis conditions of Pennisetum sp. cellulose

The present study analyzed the effects of five factors (enzymatic time,enzyme dosage,substrate concentration,buffer pH and reaction temperature) on enzymatic reaction of Pennisetum sp.cellulose.The results showed that the effects of these different factors were different and the activity of cellulose enzyme decreased as follows order: enzyme dosageenzymolysis timesubstrate concentrationbuffer pHtemperature.The optimal enzymolysis condition of Pennisetum sp.was 60 h reaction time,44.8 U·g-1 enzyme dosage,10 g·L-1 substrate concentration,pH 5.0 and 45 ℃ hydrolysis temperature.Under these optimal conditions,enzymatic hydrolyzate of sugar content was 102.656 mg·g-1.These results conducted a preliminary exploration for Pennisetum sp.as bio-ethanol fuel.