Study on the plant regeneration of creeping bentgrass

The mature seeds of 3 cultivars of Agrostis stolonifera (PennA1,Seaside and Putter) were used as the explants to study the callus induction, differentiation and rooting. The results showed that the rate of callus induction of Putter was higher than PennA1 and Seaside on MS medium supplemented with different concentrations of 2,4D. MS medium supplemented with 2 mg/L 2,4D and 0.1 mg/L 6BA was the best callus induction medium for PennA1 and Seaside, meanwhile, MS medium supplemented with 3 mg/L 2,4D and 0.1 mg/L 6BA was the best for Putter. Callus inducement rates were 45.2%, 76.5% and 87.0% respectively. MSO medium was suitable for differentiation and the callus differentiation rates were 59.0%, 50.0% and 57.6% respectively. The optimum condition for rooting was 1/2 MS+1.0 mg/L IBA.