Research progress in Medicago spp. tissue culture

There were many successes on tissue culture of Medicago spp., and the reports on M.sativa were the most. The paper presented the research progress on tissue culture of Medicago spp.in terms of selection of explants, medium composition, callus and somatic embryos induction, plant regeneration, selection of genotypes. Moreover, the existing problems and development tendency were summarized to provide useful advices on gene and cell engineering in Medicago spp.