Construction of eukaryotic expression vector bearing the heat shock factor in Carex rigescens

Based on the characteristics of the expression vector PBI 121, and its enzyme sites, the appropriate primers were designed. Using the expression primers, we amplified the Escherichia coli bearing the cDNA with CrHsf of Rigens Sedge, and the open reading frame containing the right enzyme sites of CrHsf was got. The expression vectors and the aimed fragments were digested respectively, and then, the aimed DNA fragments were cloned into PBI 121 vector. Through PCR, double enzyme restriction analysis, it was confirmed that the PBI 121/HSF recombinant plasmid was successfully constructed.