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A study on transformation of Hypodermin A gene into Medicago sativa[J]. Pratacultural Science, 2011, 5(11): 1936-1940.
Citation: A study on transformation of Hypodermin A gene into Medicago sativa[J]. Pratacultural Science, 2011, 5(11): 1936-1940.

A study on transformation of Hypodermin A gene into Medicago sativa

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  • Published Date: November 14, 2011
  • The high efficiency regeneration and genetic transformation system of alfalfa (Medicago sativa)was established by using the cotyledons as explants from the sterile 7days old seedlings of M.sativa cv. Gannong No.1. The optimal medium for callus induction and regeneration from Gannong No.1 cotyledons was MS+2,4D 2.0 mg/L+6BA 0.5 mg/L and MS+6BA 0.5 mg/L+NAA 0.03 mg/L+GA3 2.0 mg/L respectively. In this study, the efficiency transformation system included agrobacterium concentration OD6000.5, infection time 8-10 min and coculture time 2 d. The transgenic plants were identified by PCR and Southern blot techniques. The results indicated that the Hyperdomin A (HA) gene had been transformed into the genome of Gannong No.1 alfalfa.
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