Screening and identification of yeast for tolerance to high temperature, alcohol and low acid
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Graphical Abstract
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Abstract
Yeast from the different samples such as the polluted water, soil and slightly rot fruit and so on was enriched, separated, purified and selected, and 4 yeast strains for tolerance to high temperatures, low acid and alcohol, named JM-1,JM-2,JM-3 and JM-4, were obtained. The results showed that the best suitable to five-carbon sugar concentration, alcohol, acidity and temperature of 4 yeast strains were 12 g·L-1, 14% (volume ratio), between 2.5 to 3.0 as well as 37 ℃ by morphological and physiological characterization respectively. The results indicated that strains JM-1, JM-2 and JM-4 belonged to the genus Meyerozyma, and strain JM-3 belonged Pichia using sequence analysis of 26S rDNA D1/D2 region. Selected yeast strains expressed good quality and performance on behalf of tolerance to high temperature, alcohol, low acid, and had some research significance.
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