Cloning and expression analysis of CBL genes from Chrysanthemum lavandulifolium

Four ESTs encoding CBL from Chrysanthemum lavandulifolium were identified in our previous study.Four CBL genes were isolated from C.lavandulifolium and named after ClCBL1-4 using RACE method. The multiple sequence alignment analysis of Arabidopsis thaliana and C. lavandulifolium CBL sequences suggested that all of the ClCBL1-4 proteins coding sequence contained four EF-hand motifs and the ClCBL1 protein coding sequence had N-terminal myristoylation sequence. The expression patterns of ClCBLs were analysis under salt,drought,cold,heat and ABA treatments as well as under normal conditions in different developmental stages. The results suggested that ClCBLs might play important roles in C. lavandulifolium’s responses to abiotic stresses because ClCBLs can be induced under different stresses.