Effects of drought stress on chloroplast ultrastructure and physiological characteristics of <i>Seriphidium transiliense</i> seedlings

HAN Zhe; ZHANG Yongqiang; ZHANG Haohao; LUO Wenhao; CHEN Aiping

doi:10.11829/j.issn.1001-0629.2024-0116

Pratacultural Science > 2025 > Uncorrected proof > DOI: 10.11829/j.issn.1001-0629.2024-0116

HAN Z, ZHANG Y Q, ZHANG H H, LUO W H, CHEN A P. Effects of drought stress on chloroplast ultrastructure and physiological characteristics of Seriphidium transiliense seedlings. Pratacultural Science, 2025, 42(6): 1-11. DOI: 10.11829/j.issn.1001-0629.2024-0116

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Effects of drought stress on chloroplast ultrastructure and physiological characteristics of Seriphidium transiliense seedlings

HAN Zhe^{1, 2, 3,},
ZHANG Yongqiang^{1, 2, 3},
ZHANG Haohao^{1, 2, 3},
LUO Wenhao^{1, 2, 3},
CHEN Aiping^{1, 2, 3, ,}

1.
College of Grassland Science, Xinjiang Agricultural University, Urumqi 830052, Xinjiang, China
2.
Ministry of Education Key Laboratory for Western Arid Region Grassland Resources and Ecology, Urumqi 830052, Xinjiang, China
3.
Xinjiang Key Laboratory of Grassland Resources and Ecology, Urumqi 830052, Xinjiang, China

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Corresponding author:
CHEN Aiping　E-mail: xjauchenaiping@sina.com
Received Date: February 20, 2024
Accepted Date: May 05, 2024
Available Online: April 01, 2025

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Abstract

Abstract

Drought stress is a common abiotic factor affecting plant growth and development. It has severely impacted the growth and development of Seriphidium transiliense with climate change and increasingly frequent extreme weather events. In this study, we conducted a continuous drought pot experiment on S. transiliense seedlings to analyze changes in chloroplast ultrastructure, reactive oxygen species (ROS), malondialdehyde (MDA), osmotic adjustment substances, antioxidant enzyme activity, and endogenous hormone content under drought stress. The results showed that as drought stress severity increased, chloroplast ultrastructure was progressively damaged: Granal lamellae became blurred, osmiophilic particles increased, starch granules initially increased and then decreased, and cell wall separation occurred. Under severe stress, chloroplasts disintegrated and ruptured. Physiological responses exhibited a significant (P < 0.05) increase in oxygen free radicals and MDA content in leaves and roots. The levels of proline (Pro), soluble sugars (SS), and antioxidant enzyme activity initially increased and then declined in both leaves and roots. However, soluble protein (SP) content followed a different pattern, initially increasing and then decreasing in leaves, while it gradually increased in roots. The endogenous hormone abscisic acid (ABA) initially increased and then decreased in both leaves and roots, whereas indole-3-acetic acid (IAA) showed a steady decline. In conclusion, as drought stress severity increases, S. transiliense seedlings accumulate osmotic adjustment substances, enhance antioxidant enzyme activity, and regulate endogenous hormone levels to mitigate oxidative stress and maintain cell membrane stability, thereby improving drought resistance.
- drought stress,
- S. transiliense seedlings,
- chloroplast ultrastructure,
- physiological characteristics,
- drought resistance ability

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References

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Effects of drought stress on chloroplast ultrastructure and physiological characteristics of Seriphidium transiliense seedlings

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