Study on the <i>in vitro</i> fermentation characteristics and three cellulolytic bacteria of grass fiber from different species and grain size

CAO Pengfei; WANG Shuiping; TANG Jia; TANG Shaoxun; TAN Zhiliang

doi:10.11829/j.issn.1001-0629.2024-0034

Pratacultural Science > 2025 > Accepted Manuscript > DOI: 10.11829/j.issn.1001-0629.2024-0034

CAO P F, WANG S P, TANG J, TANG S X, TAN Z L. Study on the in vitro fermentation characteristics and three cellulolytic bacteria of grass fiber from different species and grain size. Pratacultural Science, 2025, 42(0): 1-17. DOI: 10.11829/j.issn.1001-0629.2024-0034

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Study on the in vitro fermentation characteristics and three cellulolytic bacteria of grass fiber from different species and grain size

CAO Pengfei^{1, 2,},
WANG Shuiping^1, ,,
TANG Jia³,
TANG Shaoxun^{2, 4, ,},
TAN Zhiliang²

1.
College of Animal Science, Southwest University, Chongqing 402460, China
2.
Key Laboratory of Agro-Ecological Processes in Subtropical Regin, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, Hunan, China
3.
Zhangjiajie Jiyuan Biotechnology Co., Ltd, Zhangjiajie 427099, Hunan, China
4.
College of Advanced Agricultural Sciences, Univeristy of Chinese Academy of Science, Beijing 100039, China

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Received Date: January 15, 2024
Accepted Date: April 07, 2024
Available Online: April 07, 2025

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Abstract

Abstract

Fiber is an important part of grass. Understanding grass fiber degradation in ruminants is beneficial to improve their production efficiency. The consistency of digestion and degradation for different grass fibers in ruminants is unclear. Therefore, here, the effects of the grass fiber source and its particle size on in vitro fermentation and the interface characteristics of microorganisms were studied. Two kinds of forage grass (alfalfa and ‘Guimu No. 1’) were selected. Neutral detergent fiber (NDF) was extracted and crushed into 3 kinds of granules: < 0.15 mm (PS1), 0.15～0.3 mm (PS2), and 0.9～1.2 mm (PS3). The experiment was divided into 6 treatment groups according to fiber source and particle size, with three replicates per treatment. The gas production parameters, fiber degradation rate, fermentation parameters, microbial interface characteristics, and the copy number of fiber-degrading bacteria and total bacteria were determined. The results showed that 1) the maximum gas production (V_f), gas production curve constant (b), time used to reach half of the maximum gas production (T_0.5), fermentation gas production fraction (FRDt_0.5), ammonia nitrogen (NH₃-N) concentration, pH, total volatile fatty acids (TVFA), and molar ratio of propionic acid in the Guimu No. 1 group were significantly higher than those in the alfalfa group (P < 0.05). In the alfalfa group, the initial gas production fraction (FRD₀), dry matter degradability (DMD), microbial protein (MCP), molar percentage of acetic acid, valeric acid, isobutyric acid, and isovalerate, and the ratio of ethylene to propylene, cell membrane permeability (CMP), liquid surface tension (ST), Fibrobacter succinogenes, Ruminococcus flavefaciens, and total bacteria were significantly higher than those in the Guimu No. 1 group (P < 0.05). 2) The b value, T_0.5, NH₃-N, and isobutyric acid molar ratio of grass fiber during fermentation tended to increase with the increase of particle size, while the FRD₀, DMD, CMP, and Ruminococcus flavefaciens significantly decreased with the increase of particle size (P < 0.05), and CSH first decreased and then increased with the increase of particle size. In summary, different grass fibers and their grinding sizes affect fiber fermentation and microbial interface characteristics in vitro. Alfalfa has a better fermentation effect than that of ‘Guimu No. 1’ and better fermentation of fiber with small grain sizes.
- alfalfa,
- ‘Guimu No.1’,
- neutral detergent fiber,
- gas production parameters,
- volatile fatty acids,
- microorganisms,
- interface characteristics

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References

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