Effects of storage years on antioxidant properties of embryonic cells of Bothriochloa ischaemum seeds from different population

To study the effects of storage years on antioxidant properties of embryonic cells of Bothriochloa ischaemum seeds from different population, five populations of B. ischaemum seeds from Daixian, Pinglu, Pingding, Liulin, Taigu of Shanxi Province were collected in 2017, 2018, 2019, and 2020, and the activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), glutathione reductase (GR), and malondialdehyde (MDA) contents were analyzed. The results showed that the MDA content in five populations of B. ischaemum seeds all increased significantly (P < 0.05). The MDA content in B. ischaemum seeds from Taigu population had the slowest change, and it only increased on the 4th storage year (P < 0.05). However, the activities of SOD, CAT, APX, and GR had a varying degree of decrease. The activities of SOD, APX, and GR showed a significant decrease on the 2nd storage year in B. ischaemum seeds from Daixian, Pinglu, Pingding, and Liulin populations (P < 0.05). In conclusion, there are different storage tolerance in B. ischaemum seeds among different populations. B. ischaemum seeds of Taigu population had the best storage tolerance, because it can be stored approximately three years; however, seeds of Daixian, Pinglu, Pingding, and Liulin populations could be stored for approximately one year.