Welcome Pratacultural Science,Today is 2025-5-2 Friday!
| Citation: |
LU M M, XIE M F, BAI B Q, SUI S Z, MA J. Ethyl methanesulfonate mutagenesis and mutant screening of two cold-season turfgrasses. Pratacultural Science, 2024, 41(4): 810-821. DOI: 10.11829/j.issn.1001-0629.2022-0973
|
This study aimed to determine the optimal mutagenesis conditions for ethyl methane sulfonate (EMS) mutagenesis treatment of Lolium perenne ‘Accent’ and Festuca arundinacea ‘Equator’ seeds and to obtain mutant material and progeny with stable traits. Seeds of both turfgrasses were mutagenized using different EMS concentrations and treatment times, and mutagenesis combinations were determined according to the semi-lethal concentration. The best mutagenic effect was achieved by treating two turfgrass seeds with 0.8% EMS solution for 36 h and then sowing them with germination rates close to 50%. After large-scale mutagenesis with these parameters, 933 mutant plants of two M1 generation turfgrass species were transplanted and survived, and a series of 18 drought-tolerant monocultures were screened after short-term drought stress. Morphological analysis of the M2 generation mutant plants revealed two mutation types: foliar narrowing and root length growth. Amplification of 20 turfgrass samples with five SCoT marker primers showed a polymorphism ratio of 84.09%. Among them, the SCoT-35 primer amplification product had the clearest and most stable bands, and the difference between the mutant and wild type was obvious. After the M2 generation of three-leaf stage mutant plants was treated with drought and high temperature stress, the malondialdehyde content, proline content, and relative conductivity all tended to increase, and the chlorophyll content tended to decrease. Two L. perenne drought-tolerant mutants A5 and A8; one F. arundinacea drought-tolerant mutant B9; two heat-tolerant mutants A8 and B9; and one drought and heat-tolerant mutant B-9 were finally obtained. The results of this study provide help for the breeding of resistant turfgrass varieties.
| [1] |
HUANG B R, DACOSTA M, JIANG Y W. Research advances in mechanisms of turfgrass tolerance to abiotic stresses: From physiology to molecular biology. Critical Reviews in Plant Sciences, 2014, 33(2/3): 141-189.
|
| [2] |
李争艳, 徐智明. 牧草和草坪草耐热性研究进展. 草业科学, 2010, 29(9): 128-133.
LI Z Y, XU Z M. Research progress on heat tolerance of forage and turfgrass. Pratacultural Science, 2010, 29(9): 128-133.
|
| [3] |
王艺程, 张世杰, 丁寒雪, 蒋成娣, 张浠然, 张志国, 刘翔. 甲基磺酸乙酯(EMS)在植物诱变育种中的应用. 分子植物育种, 2022, 12(10): 1-8.
WANG Y C, ZHANG S J, DING H X, JIANG C D, ZHANG X R, ZHANG Z G, LIU X. Application of ethyl methanesulfonate (EMS) in plant mutation breeding. Molecular Plant Breeding, 2022, 12(10): 1-8.
|
| [4] |
谭义川, 柯永培, 何雪萍, 徐学杰, 徐敏, 石海春. 19份玉米EMS诱变系的抗旱性评价. 干旱地区农业研究, 2016, 34(2): 152-156.
TAN Y C, KE Y P, HE X P, XU X J, XU M, SHI H C. Comprehensive evaluation on draught resistance of 19 maize inbred lines by EMS mutagenesis. Agricultural Research in Arid Areas, 2016, 34(2): 152-156.
|
| [5] |
VAID N, SAMUEL M A, KAGALE S, SOOLANAYAKANAHALLY, R. Chemical and Physical mutagenesis approaches for identification of herbicide and drought tolerance traits in wheat. Accelerated Breeding of Cereal Crops, 2022,: 167-179.
|
| [6] |
JIAO Y, BURKE J, CHOPRA R, BUROW G, CHEN J P, WANG B , HAYES C, EMENDACK Y, WARE D, XIN Z G. A sorghum mutant resource as an efficient platform for gene discovery in grasses. Plant Cell, 2016, 28(7): 1551.
|
| [7] |
乔雨, 石凤翎, 熊梅, 张凯旋. EMS诱变对缘毛雀麦和直立型扁蓿豆种子萌发的影响. 种子, 2016, 35(3): 98-100.
QIAO Y, SHI F L, XIONG M, ZHANG K X. Effect of EMS mutagenesis on seed germination of Brommus ciliatus L. and Medicago ruthenica L. Sojak cv. Zhilixing. Seed, 2016, 35(3): 98-100.
|
| [8] |
刘莉, 李培英. 新农1号狗牙根胚性愈伤EMS诱变初探. 新疆农业大学学报, 2016, 39(4): 273-276.
LIU L, LI P Y. Preliminary study on Ethylmethylsulfone mutation on embryonic Callus of Cynodon dactylon. (L.) Per cv. Xingnong No. 1. Journal of Xinjiang Agricultural University, 2016, 39(4): 273-276.
|
| [9] |
臧辉. 羊草种子EMS诱变效应初步研究. 北京: 中国农业科学院硕士学位论文, 2018.
ZANG H. Preliminary study on EMS mutagenesis effect on seed of Leymus chinensis. Master Thesis. Beijing: Chinese Academy of Agricultural Sciences, 2018.
|
| [10] |
COLLAED B, MACKILL D. Start codon targeted (SCoT) polymorphism: A simple, novel DNA marker technique for generating gene-targeted markers in plants. Plant Molecular Biology Reporter, 2009, 27(1): 86-93. doi: 10.1007/s11105-008-0060-5
|
| [11] |
岳庆春, 章辰飞, 汪庆昊, 王文静, 王锦阳, 吴月燕. 基于SCoT分析‘醉金香’葡萄及其突变体遗传多样性. 分子植物育种, 2020, 18(13): 4417-4426.
YUE Q C, ZHANG C F, WANG Q H, WANG W J, WANG J Y, WU Y Y. Analysis of genetic diversity of ‘Zui Jinxiang’ grape and its mutants by SCoT markers. Molecular Plant Breeding, 2020, 18(13): 4417-4426.
|
| [12] |
王俏君. 彩叶芋EMS诱变材料的SCoT分子标记鉴定及抗寒性分析. 重庆: 西南大学硕士学位论文, 2018.
WANG Q J. Identification of SCoT molecular markers and analysis of cold resistance in EMS mutagenized materials of Caladium bicolor. Master Thesis. Chongqing: Southwest University, 2018.
|
| [13] |
李建龙. 草坪草抗性生理与生态研究进展. 南京: 南京大学出版社, 2017.
LI J L. Advances in Physiological and Ecological Research of Turfgrass Resistance. Nanjing: Nanjing University Press, 2017.
|
| [14] |
李娟宁, 吕英, 赵桂琴, 柴继宽, 蔺豆豆, 王苗苗. 化学诱变剂EMS和MNU对燕麦种子萌发和幼苗生长的影响. 草原与草坪, 2021, 41(3): 108-118.
LI J N, LYU Y, ZHAO G Q, CHAI J K, LIN D D, WANG M M. Effects of chemical mutagens EMS and MNU on seed germination and seedling growth of oat. Grassland and Turf, 2021, 41(3): 108-118.
|
| [15] |
陈丽丽, 吴婍, 季晓菲, 闫利军. 不同浓度EMS对老芒麦种子发芽的影响. 草学, 2018(1): 18-22.
CHEN L L, WU Q, JI X F, YAN L J. Effect of different concentration of EMS on seed germination of Elymus sibiricus. Journal of Grassland and Forage Science, 2018(1): 18-22.
|
| [16] |
董文科, 路旭平, 姜寒玉, 马晖玲. 多年生黑麦草EMS诱变与耐旱性评价. 核农学报, 2018, 32(10): 11889-1897.
DONG W, LU X P, JIANG H Y, MA H L. Evaluation of EMS mutagenesis and drought tolerance in perennial ryegrass. Journal of Nuclear Agriculture, 2018, 32(10): 11889-1897.
|
| [17] |
潘高, 张合平, 刘鹏, 刘文胜. 锰胁迫对苍耳种子萌发及幼苗生理生化特性的影响. 草业学报, 2017, 26(11): 157-166.
PAN G, ZHANG H P, LIU P, LIU W S. Effect of manganese stress on seed germination, seeding physiological and biochemical characteristics of Xanthium sibiricum. Acta Prataculturae Sinica, 2017, 26(11): 157-166.
|
| [18] |
杨锐, 余阳俊, 徐家炳, 陈广, 张凤兰, 孙继志, 孙天水. 花期喷盐水结合蜜蜂授粉克服大白菜自交不亲和性试验. 华北农学报, 1995(2): 77-81.
YANG R, YU Y J, XU J B, CHEN G, ZHANG F L, SUN J Z, SUN T S. Studies on techniques of spraying NaCl solution flowers combined with honeybee pollination to overcome self-incompatibility of Chinese cabbage. Acta Agriculturae Boreali-Sinica, 1995(2): 77-81.
|
| [19] |
李合生. 植物生理生化实验原理和技术. 北京: 高等教育出版社, 2000: 184-261.
LI H S. Experimental Principles and Techniques of Plant Physiology and Biochemistry. Beijing: Higher Education Press, 2000: 184-261.
|
| [20] |
龚束芳, 刘恩慧, 姜童童, 周爱民, 王金刚. 尖叶石竹种子的EMS和60Co-γ诱变. 草业科学, 2017, 34(11): 2226-2234.
GONG S F, LIU E H, JIANG T T, ZHOU A M, WANG J G. Analysis of EMS and 60Co-γ mutagenesis of Dianthus spiculifolius seeds. Pratacultural Sciences, 2017, 34(11): 2226-2234.
|
| [21] |
李颜方, 杜艳伟, 王高鸿, 赵根有, 赵晋锋. EMS诱变对晋谷21号种子萌发的影响. 种子, 2020, 39(11): 83-87.
LI Y F, DU Y W, WANG G H, ZHAO G Y, ZHAO J F. Effects of EMS mutagenesis on seed germination of rice variety Jingu 21. Seed, 2020, 39(11): 83-87.
|
| [22] |
仇婷婷, 李瑞梅, 周杨骄, 林雪君, 姚远, 刘姣, 符少萍, 胡新文, 郭建春. EMS不同浓度和处理时间对华南5号木薯种子的影响. 分子植物育种, 2019, 17(9): 2965-2973.
QIU T T, LI R M, ZHOU Y J, LIN X J, YAO Y, LIU J, FU S P, HU X W, GUO J C. Effects different of EMS concentrations and treatment time on Cassava in ‘Huanan5’ seeds. Molecular Plant Breeding, 2019, 17(9): 2965-2973.
|
| [23] |
臧辉, 武自念, 孔令琪, 张继泽, 任卫波. 甲基磺酸乙酯(EMS)诱变对羊草种子萌发及幼苗生长的影响. 分子植物育种, 2018, 16(17): 5765-5769.
ZANG H, WU Z N, KONG L Q, ZHANG J Z, REN W B. Effects of ethyl methane sulfonate (EMS) mutagenesis on seed germination and seedling growth of Leymus chinensis. Molecular Plant Breeding, 2018, 16(17): 5765-5769.
|
| [24] |
霍朋杰, 吴斌, 张宗文. 裸燕麦EMS突变体库筛选与分析. 植物遗传资源学报, 2015, 16(2): 379-384.
HUO P J, WU B, ZHANG Z W. Identification and analysis of EMS mutant library in oat (Avenanuda L.). Journal of Plant Genetic Resources, 2015, 16(2): 379-384.
|
| [25] |
SAMADI N, NAGHAVI M R, MORATALLA L N, ALONSO G L, SHOKRPOUR M. Morphological, molecular and phytochemical variations induced by colchicine and EMS chemical mutagens in Crocus sativus L. Food Chemistry: Molecular Sciences, 2022, 4: 100086. doi: 10.1016/j.fochms.2022.100086
|
| [26] |
FENG S G, H, JIANG M Y, LU J J, SHEN X X, LIU J J, WANG Z A, WANG H Z. Genetic diversity and relationships of medicinal Chrysanthemum morifolium revealed by startcodon targeted (SCoT) markers. Scientia Horticulturae, 2016, 201: 118-123. doi: 10.1016/j.scienta.2016.01.042
|
| [27] |
王竞红, 陈鹏, 陈艾, 田静瑶. MUHAMMAD S, 李彦雪. 3种观赏草苗期对干旱胁迫的响应及抗旱性评价. 草业科学, 2019, 36(5): 1266-1274.
WANG J H, CHEN P, CHEN A, TIAN J Y, MUHAMMAD S, LI Y X. Effects of drought stress on physiological and growth characteristics of the seedlings of three ornamental grasses. Pratacultural Science, 2019, 36(5): 1266-1274.
|
| [28] |
赵娜, 徐庆国, 苏鹏, 梁东鸣, 唐瑶. 10个冷季型草坪草品种在高温胁迫下的抗性. 草业科学, 2019, 36(7): 1743-1753.
ZHAO N, XU Q G, SU P, LIANG D M, TANG Y. Differences in resistance to high temperature stress of 10 cold-season turfgrass varieties. Pratacultural Science, 2019, 36(7): 1743-1753.
|
| [29] |
蔺亚平, 杨成行, 苏家豪, 马清, 包爱科. 6种高寒禾草对干旱胁迫的生理响应及抗旱性评价. 草业科学, 2021, 38(12): 2397-2405.
LIN Y P, YANG C H, SU J H, MA Q, BAO A K. Physiological response of six alpine grass varieties to drought stress and their drought resistance evaluation. Pratacultural Science, 2021, 38(12): 2397-2405.
|
You are thevisitor, welcome!
Supervisor： China Association for Science and Technology
Cosponsored by： Chinese Grassland Society
College of Pastoral Agriculture Science and Technology, Lanzhou University
Post Code：730020 Tel：0931-8912486 E-mail：cykx@lzu.edu.cn Address：
Copyright © 2010 Editorial Office of 《Pratacultural Science》
Supported by Beijing Renhe Information Technology Co.Ltd. Technical ：info@rhhz.net 
DownLoad: