Cloning and functional validation of RtVAMP2-2 gene of Reaumuria trigyna

Reaumuria trigyna is a rare recretohalophyte. Its unique salt-gland structure plays a key role in its adaptation to saline desert environment, and vesicular trafficking is involved in the salt secretion process of the plant. In this study, RtVAMP2-2 gene involved in membrane vesicle trafficking was cloned based on the analysis of transcriptome data of R. trigyna under salt stress. The ORF of RtVAMP2-2 was 1 074 bp and encoded 357 amino acids. RtVAMP2-2 was localized to the plasma membrane, and its expression was induced by saline stress. Then RtVAMP2-2 was transferred into Arabidopsis thaliana for functional validation. The results showed that the transgenic Arabidopsis thaliana showed a salt-sensitive phenotype, and was speculated that the RtVAMP2-2 may have a negative regulatory effect on plant salt tolerance.