Effects of different dietary nitrogen to sulfur ratios on the jejunum tissue morphology and microflora of Tibetan lamb

YAN Xiaolan; HOU Shengzhen; LI Pengxiang; WANG Zhiyou; YANG Baochun; JIA Jianlei

doi:10.11829/j.issn.1001-0629.2020-0467

Pratacultural Science > 2021 > 38(4): 758-768. > DOI: 10.11829/j.issn.1001-0629.2020-0467

YAN X L, HOU S Z, LI P X, WANG Z Y, YANG B C, JIA J L. Effects of different dietary nitrogen to sulfur ratios on the jejunum tissue morphology and microflora of Tibetan lamb. Pratacultural Science, 2021, 38(4): 758-768 . DOI: 10.11829/j.issn.1001-0629.2020-0467

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Effects of different dietary nitrogen to sulfur ratios on the jejunum tissue morphology and microflora of Tibetan lamb

College of agriculture and animal husbandry, Qinghai University, Xining 810016, Qinghai, China

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JIA Jianlei　E-mail: 2268538152@qq.com
Received Date: September 02, 2020
Accepted Date: February 09, 2021
Available Online: April 03, 2021
Published Date: April 14, 2021

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Abstract

Abstract

The intestinal tract of animals is an important digestive and absorbing organ. To investigate the effects of different nitrogen to sulfur ratios on jejunal morphology, microbial diversity, and function in Tibetan lambs, 150 Tibetan lambs aged 60 days old with similar initial conditions were randomly divided into 5 equal groups. Lambs were fed until 180 days of age on diets containing similar energy, with nitrogen to sulfur mass ratios of 10.5 ꞉ 1 (group A), 9 ꞉ 1 (group B), 7.5 ꞉ 1 (group C), 6 ꞉ 1 (group D), and 4.5 ꞉ 1 (group E). At the end of the experiment, 6 animals from each group were randomly slaughtered and then examined. The jejunum tissues and contents were collected to analyze the effects of dietary nitrogen to sulfur ratios on jejunum tissue morphology, microbial community structure, and function. The following results were noted: 1) The length and width of jejunum villi in groups B and E were significantly higher than the other three groups (P < 0.05), and the lengths of villi in groups A and D were significantly higher than that in group C (P < 0.05). The depth of crypts in group D was significantly higher in the other four groups (P < 0.05). The villus-length/crypt-depth quotient in groups B and E was significantly higher than in groups A, C, and D (P < 0.05). The mucosal layer thicknesses in groups B and E were significantly higher than in groups C and D (P < 0.05). The muscle-layer thickness in group B was significantly higher than those of other groups (P < 0.05). 2) The OTU and Chao1 indexes of the B and E groups were significantly higher than those of the A and C groups (P < 0.05). The Shannon indexes of the B and E groups was significantly higher than those of other groups (P < 0.05). The Simpson indexes of the C and D groups were significantly higher than those of groups B and E (P < 0.05). B, D, and E group Ace indexes were significantly higher than those of A and C groups (P < 0.05). 3) Analysis of community structure of the jejunal microbial flora at phylum and genus levels revealed that at the phylum level, the Firmicutes, Actinobacteria, Bacteroidetes, and Proteobacteria predominate. Among them, the abundance of Firmicutes in group B was significantly higher than in group C (P < 0.05). The abundances of Actinobacteria in groups A and C were significantly higher than in groups B and E (P < 0.05). In groups B and E, the abundance of Bacteroidetes was significantly higher than in A and C groups (P < 0.05), and the abundance of Proteobacteria in group E was significantly higher than in groups B and D (P < 0.05). At the genus level, the four dominant bacterial genera were Rhodococcus, Brevibacterium, Christensenellaceae-R-7-group, Quinella, and Rikenellaceae-RC9-gut-group. The abundances of Rhodococcus in groups A, C, and D were significantly higher than in groups B and E (P < 0.05); the Christensenellaceae-R-7-group in group B was significantly higher than in all other groups (P < 0.05), and Quinella in groups A and B was significantly higher than in groups D and E (P < 0.05). 4) The PICRUSt function prediction results for the 16S rRNA genome show that the functions of the jejunal microbial flora mainly involve transcription and the transport and metabolism of carbohydrates and amino acids, indicating that Tibetan lamb jejunum bacteria can improve the host’s carbohydrate and protein metabolism and absorption. In summary, the nitrogen to sulfur ratio at B and D levels is beneficial to the development of jejunum tissue morphology in Tibetan lambs and increases the diversity and richness of the jejunum microbial flora. From an economic perspective, a nitrogen to sulfur ratio of 9 ꞉ 1 is most conducive to efficient raising of Tibetan lambs.
- tibetan lamb,
- nitrogen sulfur ratio,
- jejunum morphology,
- microbial diversity

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References

[1]	王慧媛, 张英杰, 刘月琴. 羊的硫营养研究进展. 中国草食动物学报, 2012(S1): 121-123. WANG H Y, ZHANG Y J, LIU Y Q. Research progress on sulfur nutrition of sheep. China Herbivores Science, 2012(S1): 121-123.
[2]	REES M C, MINSON D J. Fertilizer calcium as a factor affecting the voluntary intake, digestibility and retention time of pangola grass (Digitaria decumbens) by sheep. British Journal of Nutrition, 1978, 39(1): 5-11. doi: 10.1079/BJN19780005
[3]	张桂国, 杨在宾. 日粮不同硫水平对肉牛瘤胃发酵参数的影响. 饲料博览, 2003(11): 1-3. doi: 10.3969/j.issn.1001-0084.2003.11.001 ZHANG G G, YANG Z B. Effects of dietary sulfur levels on rumen fermentation parameters of beef cattle. Feed Review, 2003(11): 1-3. doi: 10.3969/j.issn.1001-0084.2003.11.001
[4]	殷云浩, 薛白, 阎天海, 王之盛, 刘江波. 不同氮硫比日粮对南江黄羊瘤胃体外发酵的影响. 中国畜牧杂志, 2012, 48(19): 47-50. doi: 10.3969/j.issn.0258-7033.2012.19.012 YIN Y H, XUE B, YAN T H, WANG Z S, LIU J B. Effects of different N/S ration on rumen fermentation in vitro of Nanjiang yellow sheep. Chinese Journal of Animal Science, 2012, 48(19): 47-50. doi: 10.3969/j.issn.0258-7033.2012.19.012
[5]	NRC. Nutrient Requirements of Sheep 5th Revised Edition. Washington: National Academy of Sciences, 1975.
[6]	张宏福. 动物营养参数与饲养标准. 北京: 中国农业出版社, 2010: 274-484. ZHANG H F. Nutrition Parameters and Feeding Standard for Animals. Beijing: China Agriculture Press, 2010: 274-484.
[7]	QI K, LU C D, OWENS F N. Sulfate supplementation of growing goats: Effects on performance, acid-base balance, and nutrient digestibilities. Journal of Animal Science, 1993, 71(6): 1579-1587. doi: 10.2527/1993.7161579x
[8]	王娜, 贾志海, 卢德勋, 李玉荣, 牛文艺, 娜仁, 任晓萍. 内蒙古白绒山羊日粮适宜氮硫比的综合评定研究. 动物营养学报, 1999(S1): 3-5. WANG N, JIA Z H, LU D X, LI Y R, NIU W Y, Naren, REN X P. Comprehensive evaluation of the optrogen to sulfur ration in diet of inner Mongolian white cashmere goats. Chinese Journal of Animal Nutrition, 1999(S1): 3-5.
[9]	冯媛. 用体外模拟法研究日粮氮硫比对绵羊瘤胃内环境参数、产气性能和养分降解率的影响. 沈阳: 沈阳农业大学硕士学位论文, 2016. FENG Y. Effects of dietary sulfur-nitrogen ratio on ruminal environmental parameters, gas production and nutrient degradation of sheep artificial simulation. Master Thesis. Shenyang: Shenyang Agricultural University, 2016.
[10]	姜婷婷. 外源添加木聚糖酶对幼建鲤生长性能、消化吸收能力、肠道菌群和免疫功能的影响. 成都: 四川农业大学硕士学位论文, 2013. JIANG T T. Effects of exogenous xylanase on growth performance, digestion and absorption capacity, intestinal flora and immune function of juvenile Jian carp. Master Thesis. Chengdu: Sichuan Agricultural University, 2013.
[11]	JIN J P, ZHANG L P, JIA J L, CHEN Q, ZHOU G H. Effects of maternal low-protein diet on microbiota structure and function in the jejunum of Huzhu Bamei suckling piglets. Animals, 2019, 9(10): 713. doi: 10.3390/ani9100713
[12]	蔡昕悦, 刘耀臣, 解志红, 陈应龙, 刘润进. 互惠共生微生物多样性研究概况. 微生物学通报, 2020, 47(11): 3899-3917. CAI X Y, LIU Y C, XIE Z H, CHEN Y L, LIU R J. Research advances in mutualistic symbiotic microbes diversities. Microbiology China, 2020, 47(11): 3899-3917.
[13]	于晶. 不同粘附剂, 固定液及防冰晶法对冰冻切片质量的影响. 延边: 延边大学硕士学位论文, 2017. YU J. The effect of different adhesson fixed liquid and frost crystal method on the quality of frozen slices. Master Thesis. Yanbian: Yanbian University, 2017.
[14]	闫小兰, 李鹏翔, 侯生珍, 魏涛, 贾建磊. 饲粮氮硫比对藏羔羊瘤胃发育及微生物菌群结构和功能的影响. 动物营养学报, 2021, 33(2): 974-985. YAN X L, LI P X, HOU S Z, WEI T, JIA J L. Effects of dietary nitrogen to sulfur ratio on rumen development, microbial community structure and function of tibetan lambs. Chinese Journal of Animal Nutrition, 2021, 33(2): 974-985.
[15]	景小平, 彭全辉, 胡瑞, 李倩, 周婷, 裴朝曦, 赵索南, 杨涛, 马朝银, 王之盛. 冷季补饲对藏羊小肠形态发育及营养物质转运载体基因表达量的影响. 畜牧兽医学报, 2017, 48(2): 260-271. doi: 10.11843/j.issn.0366-6964.2017.02.009 JING X P, PENG Q H, HU R, LI Q, ZHOU T, PEI Z X, ZHAO S N, YANG T, MA C Y, WANG Z S. Effects of supplementation in cold season on morphological development of small intestine and the expression of nutrient transporter gene. Chinese Journal of Animal and Veterinary Sciences, 2017, 48(2): 260-271. doi: 10.11843/j.issn.0366-6964.2017.02.009
[16]	SAMANYA M, YAMAUCHI K E. Histological alterations of intestinal villi in chickens fed dried Bacillus subtilis var. natto. Comparative Biochemistry & Physiology Part A Molecular & Integrative Physiology, 2002, 133(1): 95-104.
[17]	LINDVAL H. A novel hormone-sensitive lipase isoform expressed in pancreatic beta-cells. Journal of Biological Chemistry, 2004, 279(5): 3828-3836. doi: 10.1074/jbc.M311365200
[18]	张庆丽. 早期能量与蛋白限制饲养对1月龄断奶羔羊胃肠道发育的影响. 杨凌: 西北农林科技大学硕士论文, 2010. ZHANG Q L. Effects of early energy and protein limited feeding on gastrointestinal development of 1-month-old weaned lambs. Master Thesis. Yangling: Northwest Agriculture and Forestry University, 2010.
[19]	曲星梅, 薛复来, 黄晓瑜, 张宇, 邢晓楠, 张恩平. 断奶日龄和日粮营养水平对陕北白绒山羊小肠形态发育和消化酶活性的影响. 中国农业科学, 2019, 52(19): 3460-3470. doi: 10.3864/j.issn.0578-1752.2019.19.015 QU X M, XUE F L, HUANG X Y, ZHANG Y, XING X N, ZHANG E P. Effects of weaning age and dietary nutritional levels on intestinal morphology and activity of diges. Scientia Agricultura Sinica, 2019, 52(19): 3460-3470. doi: 10.3864/j.issn.0578-1752.2019.19.015
[20]	孙娟. 育肥方式对呼伦贝尔及呼杜杂交羔羊消化道组织形态及酶活的影响. 呼和浩特: 内蒙古农业大学硕士学位论文, 2014. SUN J. Effects of different fattening methods on digestive tract morphology and enzyme activity of Hulunbeier and Hulunbeir crossbred lambs. Master Thesis. Hohhot: Inner Mongolia Agricultural University, 2014.
[21]	STANIEY D, HUGHE R J, MOORE R J. Microbiota of the chicken gastrointestinal tract: Influence on health, productivity and disease. Applied Microbiology and Biotechnology, 2014, 98(10): 4301-4310. doi: 10.1007/s00253-014-5646-2
[22]	MAO S Y, ZHANG M L, LIU J H, ZHU W Y. Characterising the bacterial microbiota across the gastrointestinal tracts of dairy cattle: Membership and potential function. Scientific Reports, 2015, 5(1): 16116. doi: 10.1038/srep16116
[23]	BI Y L, ZENG S Q, ZHANG R, QIAO Q Y, TU Y. Effects of dietary energy levels on rumen bacterial community composition in Holstein heifers under the same forage to concentrate ratio condition. BMC Microbiology, 2018, 18(1): 69. doi: 10.1186/s12866-018-1213-9
[24]	ZHOU Z M, PEI L, MENG Q L, LI S L, CHAI S L, LIU S J, SCHONELLE J T. Assessment of ruminal bacterial and archaeal community structure in yak (Bos grunniens). Frontiers in Microbiology, 2017, 8: 179.
[25]	EVANS N J, BROWN J M, MURRY R D, GETTY B, BIRTLES R J, HART A, CARTER S D. Characterization of novel bovine gastrointestinal tract treponema isolates and comparison with bovine digital dermatitis treponemes. Applied and Environmental Microbiology, 2011, 77(1): 138-147. doi: 10.1128/AEM.00993-10
[26]	REIGSTAD C S, KASHYAP P C. Beyond phylotyping: Understanding the impact of gut microbiota on host biology. Neurogastroenterology and Motility, 2013, 25(5): 358-372. doi: 10.1111/nmo.12134
[27]	VADDER F D, KOVATCHEVA-DATCHAY P, GONCALVES D, VINERA J, MITHIEUX G. Microbiota-generated metabolites promote metabolic benefits via gut-brain neural circuits. Cell, 2014, 156(1/2): 84-96. doi: 10.1016/j.cell.2013.12.016
[28]	WANG J, FAN H, HAN Y, ZHAO T Z, ZHOU Z J. Characterization of the microbial communities along the gastrointestinal tract of sheep by 454 pyrosequencing analysis. Asian-Australasian Journal of Animal Sciences, 2017, 30(1): 100-110.
[29]	周瑞. 牛至精油对羔羊胃肠道结构和功能及其微生物多样性的影响. 兰州: 甘肃农业大学博士学位论文, 2019. ZHOU R. Effects of oregano essential oil on the structure and function of gastrointestinal tract and its microbial diversity in lambs. PhD Thesis. Lanzhou: Gansu Agricultural University, 2019.
[30]	孙渭歌. 诺卡菌多重PCR及Real-time PCR检测方法的建立. 郑州: 郑州大学硕士学位论文, 2014. SUN W G. Establishment of multiplex PCR and real time PCR for detection of Nocardia. Master Thesis. Zhengzhou: Zhengzhou University, 2014.
[31]	邱孜博, 汪荣, 张杨, 吴茜, 谢笔钧, 杨季芳, 陈吉刚, 孙智达. 红球菌及其生物降解作用研究进展. 食品科学, 2016, 37(7): 254-258. doi: 10.7506/spkx1002-6630-201607045 QIU Z B, WANG R, ZHANG Y, WU Q, XIE B J, YANG J F, CHEN J G, SUN Z D. Recent progress in studies of Rhodococcus and its application of in biodegradation. Food Science, 2016, 37(7): 254-258. doi: 10.7506/spkx1002-6630-201607045
[32]	张锐, 许晓燕, 孟尧, 卢戌, 武翠玲, 姚明明, 孟延发. 短杆菌属产胆固醇氧化酶的发酵条件优化. 中国酿造, 2006, 25(6): 23-28. doi: 10.3969/j.issn.0254-5071.2006.06.007 ZHANG R, XU X Y, MENG Y, LU X, WU C L, YAO M M, MENG Y F. Fermentation production of cholesterol oxidase by Brevibacterium sp. China Brewing, 2006, 25(6): 23-28. doi: 10.3969/j.issn.0254-5071.2006.06.007
[33]	PITTA D W, PINCHAK E W, DOWD S E, OSTERSTOCK J, GONTCHAROVA V, YOUN E, DORTOU K, YOON I, MIN B R, FULFORD J D, WICKERSHM T A, MALINOWSKI D P. Rumen bacterial diversity dynamics associated with changing from bermudagrass hay to grazed winter wheat diets. Microbial Ecology, 2010, 59(3): 511-522. doi: 10.1007/s00248-009-9609-6

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