Screening, identification, and characteristics of two phosphate-solubilizing bacteria in the rhizosphere

CHEN Jiayi; XU Jingxiu; CHEN Ziyin; CHEN Minwei; XU Ying’ao; FANG Yong

doi:10.11829/j.issn.1001-0629.2020-0158

Pratacultural Science > 2020 > 37(10): 1979-1985. > DOI: 10.11829/j.issn.1001-0629.2020-0158

CHEN J Y, XU J X, CHEN Z Y, CHEN M W, XU Y A, FANG Y. Screening, identification, and characteristics of two phosphate-solubilizing bacteria in the rhizosphere. Pratacultural Science, 2020, 37(10): 1979-1985 . DOI: 10.11829/j.issn.1001-0629.2020-0158

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Screening, identification, and characteristics of two phosphate-solubilizing bacteria in the rhizosphere

1.
Geography and Environmental Sciences Academy in Zhejiang Normal University, Jinhua 321004, Zhejiang, China
2.
Agriculture and Bioengineering Academy in Jinhua Polytechnic, Jinhua 321007, Zhejiang, China

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Corresponding author:
FANG Yong　E-mail: 384923404@qq.com
Received Date: March 26, 2020
Accepted Date: July 13, 2020
Available Online: September 27, 2020
Published Date: October 14, 2020

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Abstract

Abstract

To screen and isolate bacterial strains with high phosphate solubilizing efficiency from the rice rhizosphere and enrich the resources of these phosphate-solubilizing strains, Pikovskaya (PVK) medium and National Botanical Research Institute’s phosphate (NBRIP) medium were used. The ability of Pediococcus acidilactici 1.11 to dissolve phosphorus was tested. The obtained strains were observed under a scanning electron microscope and identified with 16S rRNA sequencing. The molybdenum-antimony colorimetric method was used to examine the effect of phosphorus dissolution. Two strains, Pseudomonas chlororaphis 1.49 and P. chlororaphis 1.209 were isolated. After culturing the isolated strains 1.49, 1.209, and 1.11 in NBRIP liquid medium for 168 h, the concentrations of the dissolved phosphorus were 109.3, 54.2 and 137.0 mg·L⁻¹, respectively. Therefore, the identified strains were highly efficient phosphate solubilizers and could be used to develop bacterial fertilizer.
- phosphate-solubilizing bacteria,
- screening,
- identification,
- characteristics

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References (22)

References

[1]	杨美英, 王春红, 武志海, 于婷, 孙合美, 刘晶晶. 不同条件下两株溶磷菌溶磷量及葡萄糖脱氢酶基因表达与酶活分析. 微生物学报, 2016, 56(4): 651-663. YANG M Y, WANG C H, WU Z H, YU T, SUN H M, LIU J J. Phosphorus dissolving capability, glucose dehydrogenase gene expression and activity of two phosphate solubilizing bacteria. Acta Microbiologica Sinica, 2016, 56(4): 651-663.
[2]	王欢, 王敬敬, 徐松, 赵维, 韩一凡, 王兴彪, 黄志勇. 有机磷降解菌的筛选及其促生特性. 微生物学报, 2017, 57(5): 667-680. WANG H, WANG J J, XU S, ZHAO W, HAN Y F, WANG X B, HUANG Z Y. Screening and growth promoting characteristics of efficient organophosphate-degradation bacteria. Acta Microbiologica Sinica, 2017, 57(5): 667-680.
[3]	李海云, 牛世全, 孔维宝, 达文燕, 耿晖, 韩彩虹, 阎薇如. 猪粪堆肥中一株溶磷菌的筛选鉴定及溶磷能力初步测定. 环境科学学报, 2015, 35(5): 1464-1470. LI H Y, NIU S Q, KONG W B, DA W Y, GENG H, HAN C H, YAN W R. Screening and identification of a phosphate solubilizing strain isolated from pig manure compost and determination of its phosphate solubilizing capacity. Acta Scientiae Circumstantiae, 2015, 35(5): 1464-1470.
[4]	张巍, 冯玉杰, 胡纯国, 王鑫. 耐盐碱解磷菌的分离鉴定及解磷能力研究. 土壤通报, 2009, 40(3): 572-575. ZHANG W, FENG Y J, HU C G, WANG X. Isolation, identification and phosphate-solubilizing capability of saline-alkali tolerance PSMs. Chinese Journal of Soil Science, 2009, 40(3): 572-575.
[5]	宫安东, 朱梓钰, 路亚南, 万海燕, 吴楠楠, CHEELD DIMUNA, 龚双军, 文淑婷, 侯晓. 吡咯伯克霍尔德菌WY6-5的溶磷、抑菌与促玉米生长作用研究. 中国农业科学, 2019, 52(9): 1574-1586. doi: 10.3864/j.issn.0578-1752.2019.09.009 GONG A D, ZHU Z Y, LU Y N, WAN H Y, WU N N, CHEELO D, GONG S J, WEN S T, HOU X. Functional analysis of Burkholderia pyrrocinia WY6-5 on phosphate solubilizing, antifungal and growth-promoting activity of maize. Scientia Agricultura Sinica, 2019, 52(9): 1574-1586. doi: 10.3864/j.issn.0578-1752.2019.09.009
[6]	ZHU J, LI M, WHELAN M. Phosphorus activators contribute to legacy phosphorus availability in agricultural soils: A review. Science of the Total Environment, 2018, 612: 522-537. doi: 10.1016/j.scitotenv.2017.08.095
[7]	马文文, 姚拓, 蒲小鹏, 卢虎, 张玉霞, 王国基. 东祁连山7种禾草根际溶磷菌筛选及其溶磷特性. 草业科学, 2015, 32(4): 515-523. doi: 10.11829/j.issn.1001-0629.2014-0163 MA W W, YAO T, PU X P, LU H, ZHANG Y X, WANG G J. Screening and characteristics of phosphate-solubilizing bacteria in rhizosphere of 7 grasses on eastern Qilian Mountains. Pratacultural Science, 2015, 32(4): 515-523. doi: 10.11829/j.issn.1001-0629.2014-0163
[8]	江红梅, 殷中伟, 史发超, 刘彩月, 程明芳, 范丙全. 一株耐盐溶磷真菌的筛选、鉴定及其生物肥料的应用效果. 植物营养与肥料学报, 2018, 24(3): 728-742. doi: 10.11674/zwyf.17468 JIANG H M, YIN Z W, SHI F C, LIU C Y, CHENG M F, FAN B Q. Isolation and functional evaluation of phosphate-solubilizing fungi with salt-tolerant characteristics. Journal of Plant Nutrition and Fertilizers, 2018, 24(3): 728-742. doi: 10.11674/zwyf.17468
[9]	史发超, 殷中伟, 江红梅, 范丙全. 一株溶磷真菌筛选鉴定及其溶磷促生效果. 微生物学报, 2014, 54(11): 1333-1343. SHI C F, YIN Z W, JIANG H M, FAN B Q. Screening, identification of P-dissolving fungus P83 strain and its effects on phosphate solubilization and plant growth promotion. Acta Microbiologica Sinica, 2014, 54(11): 1333-1343.
[10]	RAMAKRISHNA W, YADAV R, LI K F. Plant growth promoting bacteria in agriculture: Two sides of a coin. Applied Soil Ecology, 2019, 138: 10-18. doi: 10.1016/j.apsoil.2019.02.019
[11]	MEENA V S, MEENA S K, VERMA J P, KUMAR A, AERON A, MISHRA P K, BISHT J K, PATTANAYAK A, NAVEED M, NOTANIYA M L. Plant beneficial rhizospheric microorganism (PBRM) strategies to improve nutrients use efficiency: A review. Ecological Engineering, 2017, 107: 8-32. doi: 10.1016/j.ecoleng.2017.06.058
[12]	詹寿发, 卢丹, 毛花英, 熊蓉露, 黄丹, 陈晔. 2株溶磷、解钾与产 IAA 的内生真菌菌株的筛选、鉴定及促生作用研究. 中国土壤与肥料, 2017, 3: 142-151. doi: 10.11838/sfsc.20170324 ZHAN S F, LU D, MAO H Y, XIONG R L, HUANG D, CHEN Y. Isolation of two phosphate potaaium-solubilizing and IAA-producing strains of endophytic fungi and their plant growth promoting function. Soil and Fertilizer Sciences in China, 2017, 3: 142-151. doi: 10.11838/sfsc.20170324
[13]	梅新兰, 闪安琪, 蒋益, 韦中, 王誉瑶, 王世梅, 沈其荣, 徐阳春, 刘建. 适应玉米的溶磷细菌筛选及其对玉米生长的影响. 土壤学报, 2016, 53(2): 502-509. MEI X L, SHAN A Q, JIANG Y, WEI Z, WANG Y Y, WANG S M, SHEN Q R, XU Y C, LIU J. Screening of phosphate-solubilizing bacteria adaptable to corn and effects of the bacteria on the growth of corn. Acta Pedologica Sinica, 2016, 53(2): 502-509.
[14]	姜瑛, 吴越, 王国文, 徐文思, 张振, 徐莉, 胡锋, 李辉信. 一株固氮解磷菌的筛选鉴定及其对花生的促生作用研究. 土壤, 2015, 47(4): 698-703. JIANG Y, WU Y, WANG G W, XU W S, ZHANG Z, XU L, HU F, LI H X. Plant growth-promoting bacterium Variovorax sp. JX14 from calcareous alluvial soil: characterization and growth promotion on peanuts. Soils, 2015, 47(4): 698-703.
[15]	鲁如坤. 土壤和农业化学分析方法. 北京: 中国农业科学技术出版社, 1999. LU R K. Chemical Analysis of Soil Agriculture. Beijing: China Agricultural Science and Technology Press, 1999.
[16]	REZAKHANI L, MOTESHAREZADEH B, TEHRANI M M, ETESAMI H, HOSSEINI H M. Phosphate-solubilizing bacteria and silicon synergistically augment phosphorus (P) uptake by wheat (Triticum aestivum L.) plant fertilized with soluble or insoluble P source. Ecotoxicology and Environmental Safety, 2019, 173: 504-513. doi: 10.1016/j.ecoenv.2019.02.060
[17]	LIU Z G, LI Y C, ZHANG S A, FU Y Q, FAN X H, PATEL J S, ZHANG M. Characterization of phosphate-solubilizing bacteria isolated from calcareous soils. Applied Soil Ecology, 2015, 96: 217-224. doi: 10.1016/j.apsoil.2015.08.003
[18]	李海云, 姚拓, 张榕, 张洁, 李智燕, 荣良燕, 路晓雯, 杨晓蕾, 夏东慧, 罗慧琴. 红三叶根际溶磷菌的筛选与培养基优化. 草业学报, 2019, 28(1): 170-179. doi: 10.11686/cyxb2018064 LI H Y, YAO T, ZHANG R, ZHANG J, LI Z Y, RONG L Y, LU X W, YANG X L, XIA D H, LUO H Q. Isolation and screening of phosphate-solubilizing bacteria from the rhizosphere of Trifolium pratense and culture medium optimization. Acta Prataculturae Sinica, 2019, 28(1): 170-179. doi: 10.11686/cyxb2018064
[19]	YOU M, FANG S M, MACDONALD J, XU J P, YUAN Z C. Isolation and characterization of Burkholderia cenocepacia CR318, a phosphate solubilizing bacterium promoting corn growth. Microbiological Research, 2020, 233: 126395. doi: 10.1016/j.micres.2019.126395
[20]	BEHERA B C, YADAV H, SINGH S K, MISHRA R R, SETHI B K, DUTTA S K, THATOI H N. Phosphate solubilization and acid phosphatase activity of Serratia sp. isolated from mangrove soil of Mahanadi river delta, Odisha, India. Journal of Genetic Engineering and Biotechnology, 2017, 15(1): 169-178. doi: 10.1016/j.jgeb.2017.01.003
[21]	王俊娟, 阎爱华, 王薇, 李继泉, 李玉灵. 铁尾矿区油松根际溶磷泛菌D2的筛选鉴定及溶磷特性. 应用生态学报, 2016, 27(11): 3705-3711. WANG J J, YAN A H, WANG W, LI J Q, LI Y L. Screening, identification and phosphate-solubilizing characteristics of phosphate-solubilizing bacteria strain D2 (Pantoea sp.) in rhizosphere of Pinus tabuliformis in iron tailings yard. Chinese Journal of Applied Ecology, 2016, 27(11): 3705-3711.
[22]	晋婷婷, 任嘉红, 刘瑞祥. 南方红豆杉根际解有机磷细菌的鉴定及其解磷特性和促生作用研究. 西北植物学报, 2016, 36(9): 1819-1827. doi: 10.7606/j.issn.1000-4025.2016.09.1819 JIN T T, REN J H, LIU X R. Identification, characterization and growth-promoting effects of an organophosphate-solubilizing bacterium from Taxus chinensis var. mairei rhizosphere. Acta Botanica Boreali-Occidentalia Sinica, 2016, 36(9): 1819-1827. doi: 10.7606/j.issn.1000-4025.2016.09.1819

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Screening, identification, and characteristics of two phosphate-solubilizing bacteria in the rhizosphere

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