High-frequency regeneration system of mature embryos of <i>Elytrigia elongata</i>

ZHOU Yantong; GUO Qiang; MAO Peichun; TIAN Xiaoxia; CUI Guowen; MENG Lin

doi:10.11829/j.issn.1001-0629.2018-0419

Pratacultural Science > 2019 > 36(5): 1317-1322. > DOI: 10.11829/j.issn.1001-0629.2018-0419

ZHOU Y T, GUO Q, MAO P C, TIAN X X, CUI G W, MENG L. High-frequency regeneration system of mature embryos of . Pratacultural Science, 2019, 36(5): 1317-1322 . DOI: 10.11829/j.issn.1001-0629.2018-0419

Citation:

ZHOU Y T, GUO Q, MAO P C, TIAN X X, CUI G W, MENG L. High-frequency regeneration system of mature embryos of . Pratacultural Science, 2019, 36(5): 1317-1322 . DOI: 10.11829/j.issn.1001-0629.2018-0419

Citation:

ZHOU Y T, GUO Q, MAO P C, TIAN X X, CUI G W, MENG L. High-frequency regeneration system of mature embryos of . Pratacultural Science, 2019, 36(5): 1317-1322 . DOI: 10.11829/j.issn.1001-0629.2018-0419

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High-frequency regeneration system of mature embryos of Elytrigia elongata

1.
Beijing Research and Development Center for Grass and Environment, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
2.
College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, Heilongjiang, China

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Corresponding author:
MENG Lin　E-mail: menglin9599@sina.com
Received Date: July 09, 2018
Accepted Date: September 18, 2018
Available Online: November 23, 2018
Published Date: April 30, 2019

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Abstract

Abstract

The mature embryos of Elytrigia elongata seeds were used as explants and MS was used as the basic medium to carry out research on callus induction, green plantlet differentiation, and rooting by adding different growth regulator media. The results showed that the suitable medium for callus induction was MC (MS + 30 g·L^–1 Maltose + 1 g·L^–1 CH + 5 mL·L^–1 200× VB + 0.5 g·L^–1 L-Pro + 3 g·L^–1 Phytagel) + 3 mg·L^–1 2,4-D + 0.025 mg·L^–1 6-BA. The induction rate was 77.78%, and the callus was visible after 4 weeks. The best differentiation medium was MC (MS + 30 g·L^–1 Maltose + 1 g·L^–1 CH + 5 mL·L^–1 200× VB + 0.5 g·L^–1 L-Pro + 3 g·L^–1 Phytagel) + 0.1 mg·L^–1 2,4-D + 3 mg·L^–1 6-BA, and the differentiation rate was 66.67%. The budding point appeared after 4 weeks, accompanied by root hair development. The best rooting medium was MR (1/2 MS + 15 g·L^–1 Maltose + 3 g·L^–1 Phytagel) + 0.5 mg·L^–1 NAA. All plantlets survived after transplanting. Therefore, differentiation tissue culture regeneration system for E. elongata was established, including mature embryos-inducing callus-green plantlet differentiation-rooting-transplantation, which lays an important foundation for further study of its molecular mechanism of resilience.
- MS,
- 2,4-D,
- 6-BA,
- NAA,
- plant growth regulators,
- tissue culture,
- plant regeneration

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References (21)

References

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High-frequency regeneration system of mature embryos of Elytrigia elongata

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