High-frequency regeneration system of mature embryos of Elytrigia elongata

The mature embryos of Elytrigia elongata seeds were used as explants and MS was used as the basic medium to carry out research on callus induction, green plantlet differentiation, and rooting by adding different growth regulator media. The results showed that the suitable medium for callus induction was MC (MS + 30 g·L^–1 Maltose + 1 g·L^–1 CH + 5 mL·L^–1 200× VB + 0.5 g·L^–1 L-Pro + 3 g·L^–1 Phytagel) + 3 mg·L^–1 2,4-D + 0.025 mg·L^–1 6-BA. The induction rate was 77.78%, and the callus was visible after 4 weeks. The best differentiation medium was MC (MS + 30 g·L^–1 Maltose + 1 g·L^–1 CH + 5 mL·L^–1 200× VB + 0.5 g·L^–1 L-Pro + 3 g·L^–1 Phytagel) + 0.1 mg·L^–1 2,4-D + 3 mg·L^–1 6-BA, and the differentiation rate was 66.67%. The budding point appeared after 4 weeks, accompanied by root hair development. The best rooting medium was MR (1/2 MS + 15 g·L^–1 Maltose + 3 g·L^–1 Phytagel) + 0.5 mg·L^–1 NAA. All plantlets survived after transplanting. Therefore, differentiation tissue culture regeneration system for E. elongata was established, including mature embryos-inducing callus-green plantlet differentiation-rooting-transplantation, which lays an important foundation for further study of its molecular mechanism of resilience.