Establishment of highly efficient and salt tolerance alfalfa rhizobia symbiotia

In order to establish the high efficient alfalfa-rhizobia symbiotia with competitive ability and genetic stability in saline soil, germination, rhizobia salt tolerance and inoculation experiments were conducted using 12 alfalfa cultivars. The results indicated that the seed salt tolerance varied greatly among cultivars. The highest of seed salt tolerant survival percent was Zhongmu No.1 and second highest of that was Xinjiangdaye and Gannong No.7 although there was no significant difference between these three research group. The highest of rhizobia salt tolerant was Ganonng.1 with the rich strains number of 2.49×106 . Zhongmu.1. of Xinjiangdaye symbiotic with rhizobia isolated from zhongmu.1 had highest salt resistance and nitrogenase activity of rhizobia and the highest efficiency with the highest aboveground biomass, stem and leaf crude protein content and nitrogenase activity.