Effect of simulation nitrogen depositions on bacterial diversity of Deyeuxia angustifolia in wetland of Sanjiang Plain

Wetland soil bacterial community structure under the simulation nitrogen depositions was studied using 16S rDNA gene sequencing. The soil samples were collected from three different nitrogen depositions in the Sanjiang Plain Field Experiment Station of the Institute of Nature & Ecology, Heilongjiang Academy Sciences. Total microbial DNA was directly extracted from soil samples and 16S rDNA genes were sequenced and analyzed by Miseq molecular technology. The results showed that both low N and high N treatment increased the bacterial diversity compared with control treatment and low N treatment had the highest diversity. The wetland bacterial community structures significantly changed under simulation N depositions. In low N treatment, high N treatment and control treatment, Acidobacteria were predominated followed by Proteobacteria. The richness of Acidobacteria decreased and the richness of Proteobacteria increased first then decreased along the deposition. Soil pH decreased with deposition, which showing acidic property and increasing the Acidobacteria distribution. Soil physicochemical properties changed by input N which resulted in the soil bacterial community composition and abundant changed. The heatmap tree based on the 16S rDNA sequences showed that different bacterial community structure differently responded on Nitrogen deposition. This research provided basic data and theory consultation for predicting the influence of future atmospheric nitrogen deposition on soil microorganism in Sanjiang Plain wetlands.