Diversity of Stylosanthes by using DUS testing and revision of testing guidelines

In order to improve the DUS testing system, through the Guidelines for the Conduct of Tests for Distinctness, Uniformity and Stability Stylosanthes, 192 germplasm of Stylosanthes were tested by 24 measured traits.We transfermed test results as code to conduct cluster analysis. And cluster analysis showed that 192 germplasm could be divided into 11 class when the similarity coefficient was 0.770. Combined with the source of the germplasm, breeding background and results of diversity analysis, explanation of six testing characteristics were to be supplement, the pod beak was to be rejected, the color of coleoptile and the wool of stipule were to be increased. And the test standard varieties were determined. Explored the revision of the guidelines for the conduct of tests for distinctness, uniformity and stability Stylosanthes, can provide more powerful basis for the examination and approval of new varieties and species identification in Stylosanthes.