Callus induction of Stylosanthes guianensis cv. Reyan No.5

Effects of hormones 6-BA and 2,4-D and different explants on callus induction of Stylosanthes guianensis cv. Reyan No.5 were studied by orthogonal design statistic analysis. The results indicated that the main factor which influenced callus induction was 6-BA and the optimal medium for callus induction was MS medium with 2.0 mg·L-1 6-BA and 0.2~0.5 mg·L-1 2,4-D. All the explants using in the study included cotyledon, euphylla, hypocotyl, the growing point tissue, epicotyl, root, respectively could induce callus. The callus inducing rate of the explants were 95% except with that root was 80%. The budding rate of the callus from the upper hypocotyl was significantly higher than the callus from the other explants.