Incubate conditions of Androsace longifolia cell suspension culture

The light green loose callus of Androsace longifolia were selected as the basic experimental materials. The effects of different hormones, sucrose concentrations, and shaker speeds on the cell dry weight on cell suspension cultures were compared. The results showed that the optimal hormone of A.longifolia suspension culture was 6-BA which had cell dry weight of 0.15 g with optimal concentration of 0.3 mg·L-1. Cell dry weight was higher when the inoculation amount of the callus was 1 g. The optimal sucrose concentration and shaker speed was 2% and 105 r·min-1, respectively. During the culturing process, the pH value decreased and then gradually increased and stabilized at the day 21 of culture. Conductivity of the culture solution declined and then increased with the increase of cell number which was lowest after 15 days culture. The optimal harvest time of the cell suspension cultures was 12 days after culturing based on the cell dry weights.