Abstract: Based on the randomized block design for five nitrogen fertilizer levels of N0, N90, N180, N270, N360. Effects of fertilizer N on the height, the stem/leaf, yield and the organic distribution of nitrogen on Sorghum hybrid in Yangzhou region of Jiangsu province were studied, at the same time the economic benefit was accounted. The results indicated that application of nitrogen fertilizer increased the economic benefit significantly. The highest increase was 6 046 yuan/hm2 in 2008. The yield and plant height increased with the increase of nitrogen fertilizer, the fertilizer of N360 was the largest amount in the first crop and the fertilizer of N270 was the largest amount in the second and third crop. However, the effects of the nitrogen fertilizer on the stem/leaf mostly perform the znd harrest stage. The total nitrogen of S. hybrid concentrated mostly on the leaf, and the high fertilizer N treatment was larger than the low fertilizer N. The contents of total nitrogen in different organs varied as the leaf＞the whole plant＞stem＞sheath.