Abstract: This paper introduced research progress of alfalfa flavonoids. It included the classification of alfalfa flavonoids, its measuring methods such as UV spectrophotometry, colorimetry, highperformance liquid chromatography; the measuring methods of flavonoids such as ultrasonic and microwave assisted extraction, and macroporous resin adsorption method; its physiological function and the role of enhancing animal antioxidant, increasing immunity and controlling cancer. Despite of various biological activity and physiological function of alfalfa flavonoids, studies on its classification, measuring methods, extraction and separation processes, usage and safety evaluation technique were not sound and need further study.