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灌溉、磷肥及AM真菌互作对紫花苜蓿地土壤养分的影响

孟翔, 刘伟, 褚皓清, 颜安, 谢开云, 孙伶俐, 赵越

孟翔,刘伟,褚皓清,颜安,谢开云,孙伶俐,赵越. 灌溉、磷肥及AM真菌互作对紫花苜蓿地土壤养分的影响. 草业科学, 2023, 40(5): 1220-1231 . DOI: 10.11829/j.issn.1001-0629.2022-0668
引用本文: 孟翔,刘伟,褚皓清,颜安,谢开云,孙伶俐,赵越. 灌溉、磷肥及AM真菌互作对紫花苜蓿地土壤养分的影响. 草业科学, 2023, 40(5): 1220-1231 . DOI: 10.11829/j.issn.1001-0629.2022-0668
MENG X, LIU W, CHU H Q, YAN A, XIE K Y, SUN L L, ZHAO Y. Effects of irrigation, phosphate fertilizers, and arbuscular mycorrhizal fungal interaction on soil nutrient content in alfalfa fields. Pratacultural Science, 2023, 40(5): 1220-1231 . DOI: 10.11829/j.issn.1001-0629.2022-0668
Citation: MENG X, LIU W, CHU H Q, YAN A, XIE K Y, SUN L L, ZHAO Y. Effects of irrigation, phosphate fertilizers, and arbuscular mycorrhizal fungal interaction on soil nutrient content in alfalfa fields. Pratacultural Science, 2023, 40(5): 1220-1231 . DOI: 10.11829/j.issn.1001-0629.2022-0668

灌溉、磷肥及AM真菌互作对紫花苜蓿地土壤养分的影响

基金项目: 新疆维吾尔自治区重点研发项目(2022B02003);国家自然科学基金(31660604)
摘要: 土壤养分是影响紫花苜蓿(Medicago sativa)优质高产的重要因素。为探究灌溉、磷肥和丛枝菌根(AM)真菌对紫花苜蓿土壤养分的影响,本研究设置调亏灌溉(RDI)和定额灌溉(QI)两个灌溉处理,磷肥0 (P0)、60 (P1)、120 (P2)和180 kg·hm−2 (P3) 4个肥力梯度,以及AM (土壤不灭AM真菌)和–AM (土壤灭AM真菌)两种处理。结果表明:定额灌溉处理提高了根际土壤和非根际土壤有效磷含量,施磷肥和AM处理均显著增加了土壤中全磷和有效磷含量;在0-20、20-40 和40-60 cm土层紫花苜蓿根际土壤有效磷含量均大于土壤有效磷含量,说明丛枝菌根真菌对紫花苜蓿根际土壤和非根际土壤有效磷含量有重要贡献。

 

English

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  • 图  1   不同处理对土壤全钾和全氮含量的影响

    QI,定额灌溉;RDI,调亏灌溉;P0、P1、P2和P3表示施磷量分别为0、60、120和180 kg·hm−2;–AM为土壤灭AM真菌处理。不同大写字母表示同一菌根真菌处理在调亏灌溉和不同磷梯度间差异显著(P < 0.05),不同小写字母表示同一菌根真菌处理在定额灌溉下不同磷梯度间差异显著(P < 0.05),不同灌溉处理和真菌处理不做比较,由配对T检验完成)。下图同。

    Figure  1.   Effects of different treatments on the content of total kalium and nitrogen in the soil

    QI, quota irrigation; RDI, regulated deficit irrigation; P0, P1, P2, and P3 indicate the phosphorus application rates were 0, 60, 120, and 180 kg·hm−2; –AM and AM refer to the different AM treatments indicate the absence or presence of AM fungi in soil, respectively. different capital letters indicate significant difference with same mycorrhizal fungal treatment under regulated deficit irrigation and different phosphorus gradients, and different lowercase letters indicate significant difference with same mycorrhizal fungal treatment under quota irrigation and different phosphorus gradients (different irrigation and fungal treatments were not compared, and are completed by paired T-tests). this is applicable for the following figures as well .

    图  2   不同处理对土壤全磷和根际土壤全磷含量的影响

    Figure  2.   Effects of different treatments on the content of total phosphorus in rhizosphere soil and non-rhizosphere soil

    图  3   不同处理对土壤速效钾和有效磷及根际土壤有效磷含量的影响

    Figure  3.   Effects of different treatments on the content of available potassium, available phosphorus and in rhizosphere and non-rhizosphere soil

    表  1   定额和调亏灌溉的实际灌溉量

    Table  1   Irrigation amount under the two methods

    序号
    No.
    时间
    Time/
    (MM-DD)
    调亏灌溉
    Regulated deficit
    irrigation/m3
    定额灌溉
    Quota
    irrigation/m3
    105-114766
    205-2080114
    306-0377107
    406-2289131
    507-075072
    607-224463
    708-196695
    809-1588125
    合计 Total541773
    下载: 导出CSV

    表  2   不同处理下土壤全钾和土壤全氮方差分析

    Table  2   Variance analysis of soil total potassium and soil nitrogen under different treatments

    项目
    Item
    df土壤全钾 Soil total potassium土壤全氮 Soil total nitrogen
    0-20 cm20-40 cm40-60 cm0-20 cm20-40 cm40-60 cm
    FPFPFPFPFPFP
    区组 Block 3 1.077 0.305 1.355 0.250 0.242 0.625 1.443 0.236 0.828 0.367 0.829 0.367
    灌溉 Irrigation (I) 1 5.970 0.018 4.227 0.045 4.174 0.047 0.109 0.743 3.087 0.085 0.017 0.895
    磷肥 Phosphate fertilizer (P) 3 1.227 0.311 0.412 0.745 0.365 0.779 0.806 0.497 2.040 0.121 0.236 0.871
    丛枝菌根真菌
    Arbuscular mycorrhizal fungi (A)
    1 0.010 0.921 0.128 0.722 0.248 0.621 0.012 0.912 2.180 0.146 0.205 0.653
    I × P 3 0.761 0.522 0.449 0.719 0.142 0.934 0.212 0.887 1.339 0.273 0.916 0.440
    I × A 1 0.278 0.601 3.604 0.064 0.076 0.783 0.025 0.876 3.956 0.053 0.050 0.823
    P × A 3 0.980 0.410 0.522 0.669 0.413 0.745 0.294 0.830 0.583 0.629 0.584 0.628
    I × P × A 3 0.266 0.850 0.592 0.623 0.088 0.966 0.253 0.859 0.277 0.842 0.643 0.591
    下载: 导出CSV

    表  3   不同处理下土壤全磷和根际土壤全磷方差分析

    Table  3   Variance analysis of soil total phosphorus and rhizosphere soil total phosphorus under different treatments

    项目
    Item
    df土壤全磷
    Soil total phosphorus
    根际土壤全磷
    Total phosphorus in rhizosphere soil
    0-20 cm20-40 cm40-60 cm0-20 cm20-40 cm40-60 cm
    FPFPFPFPFPFP
    区组 Block 3 0.027 0.871 0.929 0.400 0.514 0.280 0.929 0.340 2.291 0.137 0.125 0.725
    灌溉 Irrigation (I) 1 4.743 0.034 6.216 0.016 4.425 0.041 0.083 0.775 0.084 0.773 0.373 0.544
    磷肥 Phosphate fertilizer (P) 3 8.514 < 0.000 1 5.831 0.002 2.717 0.055 32.258 < 0.000 1 6.022 0.001 2.014 0.125
    丛枝菌根真菌
    Arbuscular mycorrhizal fungi (A)
    1 0.268 0.607 1.108 0.298 0.203 0.654 0.021 0.886 0.383 0.539 0.776 0.383
    I × P 3 4.503 0.007 0.061 0.980 0.230 0.875 4.580 0.007 0.527 0.666 0.124 0.945
    I × A 1 3.682 0.061 0.328 0.570 1.729 0.195 2.537 0.118 0.400 0.530 1.915 0.173
    P ×A 3 5.231 0.003 0.596 0.621 0.836 0.481 3.061 0.037 0.734 0.537 0.177 0.912
    I × P × A 3 0.721 0.545 0.672 0.573 1.336 0.274 1.301 0.285 2.526 0.069 0.247 0.863
    下载: 导出CSV

    表  4   不同处理下土壤速效钾、土壤有效磷和根际土壤有效磷方差分析

    Table  4   Analysis of variance of soil available potassium, soil available phosphorus in rhizosphere soil and non-rhizosphere soil under different treatments

    项目
    Item
    df土壤速效钾 Soil available potassium
    0-20 cm20-40 cm40-60 cm
    F PF PF P
    区组 Block 3 0.169 0.069 2.264 0.139 0.059 0.809
    灌溉 Irrigation (I) 1 4.026 0.051 1.493 0.228 0.040 0.842
    磷肥 Phosphate fertilizer (P) 3 2.756 0.053 3.310 0.028 20.573 < 0.000 1
    丛枝菌根真菌
    Arbuscular mycorrhizal fungi (A)
    1 10.160 0.003 0.031 0.862 0.127 0.723

    I × P
    3 0.315 0.815 0.068 0.977 7.244 < 0.000 1
    I × A
    1 1.055 0.310 0.044 0.836 9.369 0.004
    P × A
    3 3.187 0.032 0.300 0.825 0.641 0.592
    I × P × A
    3 0.270 0.847 0.046 0.987 2.374 0.082
    项目
    Item
    df 土壤有效磷 Soil available phosphorus
    0-20 cm 20-40 cm 40-60 cm
    F P F P F P
    区组 Block 3 0.509 0.290 0.038 0.846 1.888 0.176
    灌溉 Irrigation (I) 1 57.643 < 0.000 1 38.081 < 0.000 1 62.582 < 0.000 1
    磷肥 Phosphate fertilizer (P) 3 391.834 < 0.000 1 509.828 < 0.000 1 159.614 < 0.000 1
    丛枝菌根真菌
    Arbuscular mycorrhizal fungi (A)
    1 92.344 < 0.000 1 21.501 < 0.000 1 15.752 < 0.000 1
    I × P
    3 2.031 0.122 4.068 0.012 2.222 0.098
    I × A 1 12.075 0.001 6.701 0.013 2.242 0.141
    P × A
    3 8.748 < 0.000 1 0.496 0.687 0.854 0.472
    I × P × A
    3 2.008 0.126 0.092 0.964 0.333 0.801
    项目
    Item
    df 根际土壤有效磷 Soil available phosphorus in rhizosphere
    0-20 cm 20-40 cm 40-60 cm
    F P F P F P
    区组 Block 3 2.414 0.127 0.170 0.682 0.804 0.374
    灌溉 Irrigation (I) 1 270.147 < 0.000 1 53.323 < 0.000 1 65.586 < 0.000 1
    磷肥 Phosphate fertilizer (P) 3 683.734 < 0.000 1 448.780 < 0.000 1 92.372 < 0.000 1
    丛枝菌根真菌
    Arbuscular mycorrhizal fungi (A)
    1 264.592 < 0.000 1 5.279 0.026 14.875 < 0.000 1
    I × P
    3 5.336 0.003 2.079 0.116 3.125 0.035
    I × A
    1 74.654 < 0.000 1 1.085 0.303 0.454 0.504
    P × A
    3 20.391 < 0.000 1 0.349 0.790 3.000 0.040
    I × P × A
    3 12.273 < 0.000 1 0.850 0.474 1.340 0.273
    下载: 导出CSV

    表  5   不同处理下各指标相关性分析

    Table  5   Correlation analysis of each index under different treatments

    指标
    Index
    土壤全钾
    Soil total
    kalium
    土壤全氮
    Soil total
    nitrogen
    土壤全磷
    Soil total
    phosphorus
    根际土壤全磷
    Soil total phosphorus
    in rhizosphere
    土壤速效钾
    Soil available
    kalium
    土壤有效磷
    Soil available
    phosphorus
    土壤全氮 Soil total nitrogen −0.091
    土壤全磷 Soil total phosphorus 0.350** −0.124
    根际土壤全磷
    Soil total phosphorus in rhizosphere
    0.337** −0.111 0.915**
    土壤速效钾 Soil available kalium 0.397** 0.013 0.702** 0.664**
    土壤有效磷 Soil available phosphorus 0.237** 0.033 0.507** 0.488** 0.482**
    根际土壤有效磷
    Soil available phosphorus in rhizosphere
    0.224** 0.042 0.549** 0.516** 0.482** 0.955**
    下载: 导出CSV

    表  6   不同处理下各指标综合评价

    Table  6   Comprehensive evaluation of each index under different treatments

    处理
    Treatment
    土壤全氮
    Soil total
    nitrogen
    土壤全磷
    Soil total
    phosphorus
    根际土壤
    全磷
    Soil total
    phosphorus in
    rhizosphere
    土壤全钾
    Soil total
    kalium
    有效磷
    Soil available
    phosphorus
    根际
    有效磷
    Soil available
    phosphorus in
    rhizosphere
    速效钾
    Soil available
    kalium
    平均
    Mean
    排名
    Rank
    RDI + P0 + (−AM) 0.33 0.00 0.00 1.00 0.00 0.00 0.23 0.124 ⅩⅥ
    RDI + P1 + (−AM) 0.00 0.35 0.67 0.29 0.14 0.14 0.51 0.315
    RDI + P2 + (−AM) 0.45 0.76 0.77 0.71 0.41 0.36 1.00 0.654
    RDI + P3 + (−AM) 0.20 0.26 0.24 0.50 0.69 0.60 0.95 0.508
    RDI + P0 + (AM) 1.00 0.45 0.24 0.39 0.02 0.03 0.12 0.256 ⅩⅢ
    RDI + P1 + (AM) 0.24 0.83 0.60 0.57 0.21 0.20 0.30 0.448
    RDI + P2 + (AM) 0.51 0.74 1.00 0.82 0.50 0.42 0.62 0.748
    RDI + P3 + (AM) 0.44 0.01 0.25 0.47 0.78 0.70 0.23 0.529
    QI + P0 + (−AM) 0.24 0.33 0.38 0.04 0.09 0.09 0.27 0.213 ⅩⅣ
    QI + P1 + (−AM) 0.43 0.53 0.64 0.11 0.21 0.27 0.16 0.378
    QI + P2 + (−AM) 0.81 1.00 0.82 0.29 0.51 0.50 0.53 0.663
    QI + P3 + (−AM) 0.49 0.76 0.20 0.05 0.77 0.69 0.43 0.486
    QI + P0 + (AM) 0.22 0.46 0.34 0.00 0.14 0.16 0.10 0.191 ⅩⅤ
    QI + P1 + (AM) 0.36 0.63 0.49 0.36 0.39 0.36 0.27 0.452
    QI + P2 + (AM) 0.98 0.80 0.73 0.58 0.69 0.68 0.56 0.829
    QI + P3 + (AM) 0.47 0.51 0.41 0.24 1.00 1.00 0.00 0.599
    下载: 导出CSV
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文章相关
  • 通讯作者: 谢开云
  • 收稿日期:  2022-08-25
  • 接受日期:  2022-10-11
  • 网络出版日期:  2023-04-25
  • 发布日期:  2023-05-14

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