高寒地区牧草根际促生菌的筛选与特性
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参考文献
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图 1 细菌菌株牧草根际的数量及分布情况
RS：根表土壤；RP：根系表面；HP：根内组织；PGPR: 植物根际促生菌。
Figure 1. Number and distribution of bacterial strains in the rhizosphere of forage species
RS: soil adhering to roots; RP: rhizoplane or surface of roots; HP: histoplane or interior of roots; PGPR: plant growth-promoting rhizobacteria.
表 1 PGPR菌株溶解有机磷能力
Table 1 Ability of PGPR to dissolve organic phosphorus
菌株编号
Strain code溶有机磷量
Dissolved organic
phosphorus/(µg·mL−1)pH ZMBJ1 15.83 ± 1.04i 3.13 ± 0.06efg ZMBJ2 141.49 ± 0.48a 2.97 ± 0.22g ZMBJ3 93.68 ± 8.17c 3.69 ± 0.09abc ZMBB1 63.45 ± 0.29e 3.01 ± 0.05fg ZMBB2 11.36 ± 0.57j 3.31 ± 0.04def ZMBN2 69.87 ± 0.29d 3.79 ± 0.27a ZMBN4 120.96 ± 1.00b 3.72 ± 0.39ab SMCB3 73.33 ± 2.13d 3.38 ± 0.05cde GMDJ1 26.35 ± 0.29h 3.46 ± 0.06bcd GMDJ3 44.49 ± 2.19f 3.53 ± 0.12abcd GMDJ4 33.97 ± 1.50g 3.41 ± 0.20bcde GMDJ5 17.78 ± 0.82i 3.51 ± 0.06abcd GMDB1 6.51 ± 0.29k 3.32 ± 0.02def GMDB3 9.91 ± 0.29jk 3.21 ± 0.25defg 不同小写字母表示不同菌株之间差异显著(P < 0.05)；下表同。
Different lowercase letters within the same column indicate significant difference between the different strains at the 0.05 level; this is applicable for the following tables as well.
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表 2 PGPR菌株溶解无机磷能力
Table 2 Ability of PGPR to dissolve inorganic phosphorus
菌株编号
Strain code溶无机磷量
Dissolved inorganic
phosphorus/(µg·mL−1)pH ZPBJ2 417.52 ± 12.36de 4.34 ± 0.16cde ZPBJ3 417.15 ± 15.79de 3.98 ± 0.06f ZPBB1 381.24 ± 11.78f 4.52 ± 0.20bc ZPBB3 485.05 ± 20.03b 4.09 ± 0.11ef ZPBN1 533.93 ± 24.56a 4.04 ± 0.07f SPCJ2 498.65 ± 14.15b 4.03 ± 0.06cd SPCJ4 443.85 ± 5.54cd 4.19 ± 0.10def SPCB1 401.40 ± 27.90ef 4.79 ± 0.04a SPCB2 445.36 ± 24.00cd 4.56 ± 0.40abc SPCB4 470.31 ± 24.46bc 4.69 ± 0.06ab SPCN2 468.29 ± 21.88bc 4.16 ± 0.10def GPDJ1 371.29 ± 2.15f 4.36 ± 0.03cd GPDJ2 465.39 ± 3.17bc 4.04 ± 0.02f GPDB2 374.31 ± 21.21f 4.40 ± 0.19f GPDB3 538.59 ± 28.75a 3.98 ± 0.04f GPDB4 385.20 ± 16.42ef 3.99 ± 0.05f
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表 3 PGPR菌株固氮能力
Table 3 Nitrogen fixing capacity of PGPR strains
菌株编号
Strain code固氮酶活性(C2H4)
Nitrogen capacity/[nmol·(h·mL)−1]ZNBJ1 152.53 ± 12.58ab ZNBJ2 135.74 ± 17.84abcd ZNBJ3 143.63 ± 13.86abc ZNBJ4 138.96 ± 21.72abcd ZNBJ5 134.74 ± 21.39abcd ZNBJ6 143.77 ± 19.73abc ZNBB1 121.34 ± 8.43abcd ZNBB2 110.60 ± 29.44bcd ZNBB3 106.88 ± 10.80bcd ZNBN1 131.27 ± 26.22abcd SNCJ1 138.46 ± 13.78abcd SNCJ2 125.55 ± 25.42abcd SNCJ3 135.63 ± 4.70abcd SNCB2 129.15 ± 31.61abcd GNDJ1 160.20 ± 37.14a GNDJ3 101.35 ± 5.66cd GNDJ4 139.38 ± 16.02abcd GNDJ5 106.62 ± 16.08bcd GNDJ6 132.27 ± 4.95abcd GNDB2 107.42 ± 46.28bcd GNDN1 136.84 ± 17.94abcd GNDN2 91.71 ± 60.49d
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表 4 PGPR菌株分泌植物激素能力
Table 4 Ability of PGPR strains to secrete plant hormones
菌株编号
Strain code显色反应
Chromogenic reaction赤霉素(GA3)
Gibberellic acid/(µg·mL−1)生长素(IAA)
Indole-3-acetic acid/(µg·mL−1)玉米素(t-Z)
Trans-zeatin/(µg·mL−1)ZNBJ1 ++ − 0.27 ± 0.03d 0.12 ± 0.00e ZNBJ6 + − 0.10 ± 0.01d 0.14 ± 0.01de ZMBJ3 + 2.37 ± 0.03bc 0.15 ± 0.01d 0.24 ± 0.00cd ZNBB1 + − 0.12 ± 0.02d 0.49 ± 0.12b ZMBN2 + 5.16 ± 0.63bc 0.17 ± 0.04d 0.13 ± 0.01e ZMBN4 ++ 139.22 ± 17.62a 0.34 ± 0.05bc 0.18 ± 0.01cde ZPBN1 ++ 0.52 ± 0.02d 0.14 ± 0.02d 0.25 ± 0.05c SNCJ3 ++ 12.30 ± 1.49b 0.41 ± 0.06b 0.13 ± 0.01e SPCJ2 ++ 1.80 ± 0.09bc 0.13 ± 0.02d 0.12 ± 0.02e SPCB4 ++ 2.99 ± 1.30bc 0.92 ± 0.13a 0.99 ± 0.04a GMDJ3 + − 0.12 ± 0.02d 0.16 ± 0.06cde GNDJ4 + 1.00 ± 0.18bc 0.10 ± 0.002d 0.15 ± 0.02cde GMDB3 + 3.70 ± 0.19bc 0.11 ± 0.01d 0.16 ± 0.08cde GPDB3 + − 0.14 ± 0.01d 0.14 ± 0.01de IAA显色反应中；“++”表示深粉色；“+”表示浅粉色。
In the color reaction of IAA; “++” indicates dark pink; “+” indicates light pink.
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表 5 优良PGPR菌株综合特性
Table 5 Comprehensive characteristics of excellent PGPR strains
菌株编号
Strain code溶有机磷量
Dissolved organic
phosphorus/(µg·mL−1)溶无机磷量
Dissolved inorganic
phosphorus/(µg·mL−1)固氮酶活性
Nitrogen capacity/
[nmol·(h·mL)−1](C2H4)赤霉素(GA3)
Gibberellic acid/
(µg·mL−1)生长素(IAA)含量
Indole-3-acetic
acid/(µg·mL−1)玉米素(t-Z)
Trans-zeatin/
(µg·mL−1)ZMBJ2 141.40 ± 0.48a 307.60 ± 0.19ef 51.11 ± 7.52b − − − ZMBJ3 93.68 ± 8.17c 373.03 ± 0.50d 28.06 ± 15.47bc 2.37 ± 0.03bc 0.15 ± 0.01d 0.24 ± 0.00cd ZMBN4 120.90 ± 1.00b 288.50 ± 0.29f 37.68 ± 16.97bc 139.20 ± 17.62a 0.34 ± 0.05bc 0.10 ± 0.01cde ZPBN1 30.44 ± 0.50g 533.90 ± 24.57a − 0.52 ± 0.02d 0.14 ± 0.02d 0.25 ± 0.05c ZNBJ1 28.68 ± 0.58g 260.10 ± 0.66g 152.53 ± 12.58a − 0.27 ± 0.03d 0.12 ± 0.00e ZNBJ3 46.00 ± 0.48f 326.17 ± 0.57e 143.63 ± 13.86a − − − SPCJ2 47.45 ± 0.50f 498.60 ± 14.15b 18.50 ± 6.79bc 1.80 ± 0.09bc 0.13 ± 0.02d 0.12 ± 0.02e SPCB4 71.70 ± 0.29d 470.30 ± 24.46c 14.96 ± 0.47c 2.99 ± 1.30bc 0.92 ± 0.13a 0.99 ± 0.04a GPDB3 32.46 ± 0.22g 538.50 ± 28.75a 15.34 ± 1.49c − 0.14 ± 0.01d 0.14 ± 0.01de GNDJ1 70.94 ± 0.39d 292.09 ± 4.58f 160.20 ± 37.14a − − − GNDJ4 − 363.90 ± 0.85d 139.38 ± 16.02a 1.00 ± 0.18bc 0.10 ± 0.00 0.15 ± 0.02cde GNDN1 64.90 ± 0.48e 319.49 ± 8.95e 136.84 ± 17.94a − − −
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表 6 优良PGPR菌株鉴定
Table 6 Identification of superior PGPR strains
菌株编号
Strain code宿主植物
Host plant分离部位
Separation site同源性菌株
Homologous strain相似度
Similarity/%SPCB4 草地早熟禾 Poa pratensis RP Enterobacter huaxiensis 98.54 ZNBJ3 中华羊茅 Festuca sinensis RS Pseudomonas piscium 99.59 ZNBJ1 中华羊茅 Festuca sinensis RS Pseudomonas piscium 99.35 SPCJ2 草地早熟禾 Poa pratensis RS Pseudomonas piscium 99.19 GPDB3 紫穗鹅观草 Roegneria purpurascens RP Pseudomonas piscium 99.59 GNDN1 紫穗鹅观草 Roegneria purpurascens HP Pseudomonas piscium 99.59 GNDJ1 紫穗鹅观草 Roegneria purpurascens RS Pseudomonas piscium 99.51 ZPBN1 中华羊茅 Festuca sinensis HP Pseudomonas neuropathica 98.78 ZMBJ3 中华羊茅 Festuca sinensis RS Pseudomonas neuropathica 99.19 ZMBJ2 中华羊茅 Festuca sinensis RS Pseudomonas pisciculturae 99.30 ZMBN4 中华羊茅 Festuca sinensis HP Pseudomonas bubulae 98.88 GNDJ4 紫穗鹅观草 Roegneria purpurascens RS Pseudomonas mucoides 99.19 RP：根表面； RS：根表土；HP：根内。
RP: rhizoplane or surface of roots; RS: soil adhering to roots; HP: histoplane or interior of roots.
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