PEG引发紫花苜蓿种子的最优处理方案
English
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参考文献
[1] 毛培胜, 侯龙鱼, 王明亚. 中国北方牧草种子生产的限制因素和关键技术. 科学通报, 2016, 61(2): 250-260. MAO P S, HOU L Y, WANG M Y. Limiting factors and key technologies for forage seed production in northern China. Chinese Science Bulletin, 2016, 61(2): 250-260.
[2] 刘自学. 草种业现状与发展趋势. 西宁: 第四届中国草业大会论文集, 2016:347-348. LIU Z X. Grass seed industry status and development trend. Xining: Proceedings of the 4th China Grass Industry Conference, 2016:347-348.
[3] 王彦荣. 种子引发的研究现状. 草业学报, 2004, 13(4): 7-12. doi: 10.3321/j.issn:1004-5759.2004.04.002 WANG Y R. Research status of seed priming. Acta Prataculturae Sinica, 2004, 13(4): 7-12. doi: 10.3321/j.issn:1004-5759.2004.04.002
[4] HEYDECKER W, HIGGINS J, GULLIVER R L. Accelerated germination by osmoticseed treatment. Nature, 1973, 246: 42-44. doi: 10.1038/246042a0
[5] ROWSE H R. Drum priming non-srnotic method of priming seeds. Seed Science and Technology, 1996, 24: 281-294.
[6] TAYLOR A G, KLEIN D E. WHITLOW T H. Solid matrix priming of seeds. Scientia Horticulturae, 1988, 37: 1-11. doi: 10.1016/0304-4238(88)90146-X
[7] CARPENTER W J, BOUCHER J F. Priming improves high-temperature germination of pansy seed. HortScience, 1991, 26: 541-544. doi: 10.21273/HORTSCI.26.5.541
[8] FAROOQ M, BASRA S M A, SALEEM B A, NAFEES M, CHISHTI S A. Enhanceent of tomato seed germination and seedling vigor by osmopriming. Pakistan Journal of Agricultural Sciences, 2005, 42(3/4): 36-41.
[9] 闵丹丹, 范燕, 郭正刚, 胡小文. 紫花苜蓿种子水引发条件的优化. 草业科学, 2016, 33(4): 669-673. doi: 10.11829/j.issn.1001-0629.2015-0306 MIN D D, FAN Y, GUO Z G, HU X W. Optimization of seed hydropriming condition of Medicago sativa. Pratacultural Science, 2016, 33(4): 669-673. doi: 10.11829/j.issn.1001-0629.2015-0306
[10] MCDONALD M B. Seed priming. // BLACK M, BEWLEY J D. (eds). Seed Technology and Its Biological Basis. UK: Sheffield Acad, 2000: 287-325.
[11] 张晓艳, 李宇歌. PEG渗透处理对老化种子活力的影响. 吉林师范大学学报(自然科学版), 2005, 5(2): 50-52. ZHANG X Y, LI Y G. Effect of PEG osmotic treatment on the vitality of aged seeds. Journal of Jilin Normal University (Natural Science), 2005, 5(2): 50-52.
[12] 季鹏, 于靖, 张军林, 李焕宇, 吕婕. 聚乙二醇(PEG)引发种子的机制及最适引发条件的选择. 作物栽培, 2006(6): 18-20. JI P, YU J, ZHANG J L, LI H Y, LYU J. Mechanism of priming seed with polyethylene glycol (PEG) and selection of optimum priming conditions. Crop Cultivation, 2006(6): 18-20.
[13] 闵丹丹, 潘佳, 范燕, 王彦荣, 胡小文. 引发对种子萌发和幼苗生长特性的影响. 草业科学, 2016, 33(9): 1728-1738. doi: 10.11829/j.issn.1001-0629.2015-0719 MIN D D, PAN J, FAN Y, WANG Y R, HU X W. Effect of priming on seed germination and seeding growth. Pratacultural Science, 2016, 33(9): 1728-1738. doi: 10.11829/j.issn.1001-0629.2015-0719
[14] 朴锦, 具红光. 正交设计优化PEG引发关苍术种子萌发的最优处理方案. 中国农学通报, 2015, 31(7): 138-142. PIAO J, JU H G. The best treatment scheme of orthogonal design for optimizing the effects of seed soaking in PEG on germination percentage of Atractylodes japonica Koidz ex Kitam. Chinese Agricultural Science Bulletin, 2015, 31(7): 138-142.
[15] 冯文新, 韩占芳, 王玉国, 李彩霞. 钙浸种对小麦幼苗保护酶活性及膜功能的影响. 麦类作物学报, 1997(3): 31-33. FENG W X, HAN Z F, WANG Y G, LI C X. Effects of calcium soaking on the activity of protective enzymes and membrane function in wheat seedlings. Triticeae Crops, 1997(3): 31-33.
[16] BRADFORD K J. Manipulation of seed water relations via osmotic priming to improve germination under stress conditions. Hort Science, 1986, 21: 1005-1112.
[17] IBRAHIM E A. Seed priming to alleviate salinity stress in germinating seeds. Journal of Plant Physiology, 2016, 192: 38-46. doi: 10.1016/j.jplph.2015.12.011
[18] YU T, BO G, ZHOU D W. Responses of seed germination, seedling growth, and seed yield traits to seed pretreatment in maize (Zea mays L.). The Scientific World Journal, 2014(6): 1-8.
[19] Hardegree S P. Optimization of seed priming treatments to increase low-temperature germination rate. Journal of Range Management, 1996, 49(1): 87-92. doi: 10.2307/4002731
[20] 赵玥, 辛霞, 王宗礼, 卢新雄. 种子引发机理研究进展及牧草种子引发研究展望. 中国草地学报, 2012, 34(3): 102-108. doi: 10.3969/j.issn.1673-5021.2012.03.017 ZHAO Y, XIN X, WANG Z L, LU X X. Research progress and progress and progress and prospects in the mechanism of seed priming. Chinese Journal of Grassland, 2012, 34(3): 102-108. doi: 10.3969/j.issn.1673-5021.2012.03.017
[21] 郑光华. 种子生理学. 北京: 科学出版社, 2004. ZHENG G H. Seed Physiology. Beijing: Science Press, 2004.
[22] RAHIMI. Seed priming improves the germination performance of cumin (Cuminum syminum L.) under temperature and water stres. Industrial Crops & Products, 2013, 42(1): 454-460.
[23] 韩蕊莲, 候庆春, 邹厚远, 梁宗锁. 用PEG" 引发”沙打旺种子活力及抗逆性的研究. 草业科学, 1993, 10(6): 60-63. HAN R L, HOU Q C, ZOU H Y, LIANG Z S. Study of the vitality and resistance on PEG priming seed of Astragalus adurgen. Pratacultural Science, 1993, 10(6): 60-63.
[24] GALHAUT L, DE LESPINAY A, WALKER D J, BERNAL M P, CORREAL E, LUTTS S. Seed priming of Trifolium repens L. improved germination and early seedling growth on heavy metal-contaminated soil. Water, Air, & Soil Pollution, 2014, 225(4): 1905-3353.
[25] 张燕, 方力, 吴业池, 冯永新, 姚照兵. PEG浸种处理提高烟草种子活力的效应. 种子, 2003(6): 26-29. doi: 10.3969/j.issn.1000-8071.2003.06.014 ZHANG Y, FANG L, WU Y C, FENG Y X, YAO Z B. Effect of soaking seeds with PEG on improving seed vigor of tobacco. Seed, 2003(6): 26-29. doi: 10.3969/j.issn.1000-8071.2003.06.014
[26] 覃柳燕, 唐美琼, 梁莹, 李林轩, 缪剑华. 不同温度贮藏后山豆根种子酶活性与发芽率相关性研究. 种子, 2011, 30(11): 8-10. doi: 10.3969/j.issn.1000-8071.2011.11.004 QIN L Y, TANG M Q, LIANG Y, LI L X, MIU J H. Study on the relationship between antioxidant enzymes activities and germination rate of Sophora tonkinensis Gagnep seed storaged at different temperatures. Seed, 2011, 30(11): 8-10. doi: 10.3969/j.issn.1000-8071.2011.11.004
[27] REHMAN H U, IQBAL H, BASRA S M A, IRFAN A, MUHAMMAD F, ABDUL W, WANG N. Seed priming improves early seedling vigor, growth and productivity of spring maize. Journal of Integrative Agriculture, 2015, 14(9): 1745-1754. doi: 10.1016/S2095-3119(14)61000-5
[28] LI X M, Zhang L H. SA and PEG-induced priming for water stress tolerance in rice seedling. // Information Technology and Agricultural Engineering. Springer, 2010.
[29] MOHAMMAD A, ABDUL H, SHAO Y H. Seed osmopriming invokes stress memory against post-germinative drought stress in wheat (Triticum aestivum L.). Environmental and Experimental Botany, 2018, 145: 12-20. doi: 10.1016/j.envexpbot.2017.10.002
[30] 王炜, 史雨刚, 王曙光. PEG引发对老化大豆种子发芽及活力的影响. 山西农业科学, 2011, 39(7): 650-654. doi: 10.3969/j.issn.1002-2481.2011.07.07 WANG W, SHI Y G, WANG S G. Effect of PEG on germination and seed vigor of aging soybean. Journal of Shanxi Agricultural Science, 2011, 39(7): 650-654. doi: 10.3969/j.issn.1002-2481.2011.07.07
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表 1 PEG 6000引发试验因素及水平
Table 1 Factors and levels of PEG 6000 priming test
水平 Level 因素 Factor PEG浓度
PEG concentration/%处理时间
Treatment time/h处理温度
Treatment temperature/℃1 5 12 10 2 10 24 15 3 15 36 20 
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表 2 L9 (33)正交表头设计
Table 2 L9 (33) orthogonal header design
处理组合 Treatment PEG浓度
PEG concentration (A)处理时间Treatment time (B) 处理温度Treatment temperature (C) Ⅰ 1 1 1 Ⅱ 1 2 2 Ⅲ 1 3 3 Ⅳ 2 1 2 Ⅴ 2 2 3 Ⅵ 2 3 1 Ⅶ 3 1 3 Ⅷ 3 2 1 Ⅸ 3 3 2
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表 3 方差分析表
Table 3 RANOVA table
变异来源
Source of
variation离均差
平方和 SS自由度
df均方
MSF P A 1 164.437 2 582.218 38.54 0.025 B 186.634 2 93.317 6.177 0.139 C 118.573 2 59.286 3.924 0.203 误差 Error 30.214 2 15.107
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表 4 紫花苜蓿正交试验结果表
Table 4 Results of orthogonal test
序号
Serial number试验因素
Test factor水平
Level均数
Mean极差
Range95%置信区间
95% confidence interval下限
Lower limit上限
Upper limit1 63.797 61.329 66.264 1 A 2 91.603 27.806 76.298 106.909 3 79.22 47.704 110.736 1 84.527 41.681 127.373 2 B 2 78.493 12.927 41.352 115.634 3 71.6 42.675 100.525 1 72.02 44.131 99.909 3 C 2 79.963 10.617 37.459 122.468 3 82.637 41.68 123.594
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表 5 PEG处理对紫花苜蓿种子MDA含量、SOD、POD及CAT酶的活性的影响
Table 5 Effects of PEG treatment on the content of MDA, the activity of SOD, POD and CAT in alfalfa seeds
序号 Serial number 处理组合 Treatment SOD/(U·mg–1) POD/(U·mg–1) CAT/(U·mg–1) MDA/(nmol·mg–1) 对照 Control 对照 Control 535.45 ± 49.96c 284.65 ± 36.71c 34.34 ± 11.85c 445.97 ± 200.13a Ⅳ A2B1C2 1 767.83 ± 609.30b 1 457.11 ± 163.30b 621.07 ± 152.55a 153.46 ± 20.31b Ⅴ A2B2C3 2 515.92 ± 531.31a 1 787.10 ± 191.09a 420.83 ± 160.84ab 147.39 ± 25.45b Ⅵ A2B3C1 1 526.98 ± 658.40b 1 426.97 ± 132.32b 177.54 ± 98.40b 126.17 ± 29.18b 同列不同小写字母表示不同处理间差异显著(P < 0.05)。
Different lowercase letters in the same column indicate significant difference between different treatments at the 0.05 level.
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[1] 毛培胜, 侯龙鱼, 王明亚. 中国北方牧草种子生产的限制因素和关键技术. 科学通报, 2016, 61(2): 250-260. MAO P S, HOU L Y, WANG M Y. Limiting factors and key technologies for forage seed production in northern China. Chinese Science Bulletin, 2016, 61(2): 250-260.
[2] 刘自学. 草种业现状与发展趋势. 西宁: 第四届中国草业大会论文集, 2016:347-348. LIU Z X. Grass seed industry status and development trend. Xining: Proceedings of the 4th China Grass Industry Conference, 2016:347-348.
[3] 王彦荣. 种子引发的研究现状. 草业学报, 2004, 13(4): 7-12. doi: 10.3321/j.issn:1004-5759.2004.04.002 WANG Y R. Research status of seed priming. Acta Prataculturae Sinica, 2004, 13(4): 7-12. doi: 10.3321/j.issn:1004-5759.2004.04.002
[4] HEYDECKER W, HIGGINS J, GULLIVER R L. Accelerated germination by osmoticseed treatment. Nature, 1973, 246: 42-44. doi: 10.1038/246042a0
[5] ROWSE H R. Drum priming non-srnotic method of priming seeds. Seed Science and Technology, 1996, 24: 281-294.
[6] TAYLOR A G, KLEIN D E. WHITLOW T H. Solid matrix priming of seeds. Scientia Horticulturae, 1988, 37: 1-11. doi: 10.1016/0304-4238(88)90146-X
[7] CARPENTER W J, BOUCHER J F. Priming improves high-temperature germination of pansy seed. HortScience, 1991, 26: 541-544. doi: 10.21273/HORTSCI.26.5.541
[8] FAROOQ M, BASRA S M A, SALEEM B A, NAFEES M, CHISHTI S A. Enhanceent of tomato seed germination and seedling vigor by osmopriming. Pakistan Journal of Agricultural Sciences, 2005, 42(3/4): 36-41.
[9] 闵丹丹, 范燕, 郭正刚, 胡小文. 紫花苜蓿种子水引发条件的优化. 草业科学, 2016, 33(4): 669-673. doi: 10.11829/j.issn.1001-0629.2015-0306 MIN D D, FAN Y, GUO Z G, HU X W. Optimization of seed hydropriming condition of Medicago sativa. Pratacultural Science, 2016, 33(4): 669-673. doi: 10.11829/j.issn.1001-0629.2015-0306
[10] MCDONALD M B. Seed priming. // BLACK M, BEWLEY J D. (eds). Seed Technology and Its Biological Basis. UK: Sheffield Acad, 2000: 287-325.
[11] 张晓艳, 李宇歌. PEG渗透处理对老化种子活力的影响. 吉林师范大学学报(自然科学版), 2005, 5(2): 50-52. ZHANG X Y, LI Y G. Effect of PEG osmotic treatment on the vitality of aged seeds. Journal of Jilin Normal University (Natural Science), 2005, 5(2): 50-52.
[12] 季鹏, 于靖, 张军林, 李焕宇, 吕婕. 聚乙二醇(PEG)引发种子的机制及最适引发条件的选择. 作物栽培, 2006(6): 18-20. JI P, YU J, ZHANG J L, LI H Y, LYU J. Mechanism of priming seed with polyethylene glycol (PEG) and selection of optimum priming conditions. Crop Cultivation, 2006(6): 18-20.
[13] 闵丹丹, 潘佳, 范燕, 王彦荣, 胡小文. 引发对种子萌发和幼苗生长特性的影响. 草业科学, 2016, 33(9): 1728-1738. doi: 10.11829/j.issn.1001-0629.2015-0719 MIN D D, PAN J, FAN Y, WANG Y R, HU X W. Effect of priming on seed germination and seeding growth. Pratacultural Science, 2016, 33(9): 1728-1738. doi: 10.11829/j.issn.1001-0629.2015-0719
[14] 朴锦, 具红光. 正交设计优化PEG引发关苍术种子萌发的最优处理方案. 中国农学通报, 2015, 31(7): 138-142. PIAO J, JU H G. The best treatment scheme of orthogonal design for optimizing the effects of seed soaking in PEG on germination percentage of Atractylodes japonica Koidz ex Kitam. Chinese Agricultural Science Bulletin, 2015, 31(7): 138-142.
[15] 冯文新, 韩占芳, 王玉国, 李彩霞. 钙浸种对小麦幼苗保护酶活性及膜功能的影响. 麦类作物学报, 1997(3): 31-33. FENG W X, HAN Z F, WANG Y G, LI C X. Effects of calcium soaking on the activity of protective enzymes and membrane function in wheat seedlings. Triticeae Crops, 1997(3): 31-33.
[16] BRADFORD K J. Manipulation of seed water relations via osmotic priming to improve germination under stress conditions. Hort Science, 1986, 21: 1005-1112.
[17] IBRAHIM E A. Seed priming to alleviate salinity stress in germinating seeds. Journal of Plant Physiology, 2016, 192: 38-46. doi: 10.1016/j.jplph.2015.12.011
[18] YU T, BO G, ZHOU D W. Responses of seed germination, seedling growth, and seed yield traits to seed pretreatment in maize (Zea mays L.). The Scientific World Journal, 2014(6): 1-8.
[19] Hardegree S P. Optimization of seed priming treatments to increase low-temperature germination rate. Journal of Range Management, 1996, 49(1): 87-92. doi: 10.2307/4002731
[20] 赵玥, 辛霞, 王宗礼, 卢新雄. 种子引发机理研究进展及牧草种子引发研究展望. 中国草地学报, 2012, 34(3): 102-108. doi: 10.3969/j.issn.1673-5021.2012.03.017 ZHAO Y, XIN X, WANG Z L, LU X X. Research progress and progress and progress and prospects in the mechanism of seed priming. Chinese Journal of Grassland, 2012, 34(3): 102-108. doi: 10.3969/j.issn.1673-5021.2012.03.017
[21] 郑光华. 种子生理学. 北京: 科学出版社, 2004. ZHENG G H. Seed Physiology. Beijing: Science Press, 2004.
[22] RAHIMI. Seed priming improves the germination performance of cumin (Cuminum syminum L.) under temperature and water stres. Industrial Crops & Products, 2013, 42(1): 454-460.
[23] 韩蕊莲, 候庆春, 邹厚远, 梁宗锁. 用PEG" 引发”沙打旺种子活力及抗逆性的研究. 草业科学, 1993, 10(6): 60-63. HAN R L, HOU Q C, ZOU H Y, LIANG Z S. Study of the vitality and resistance on PEG priming seed of Astragalus adurgen. Pratacultural Science, 1993, 10(6): 60-63.
[24] GALHAUT L, DE LESPINAY A, WALKER D J, BERNAL M P, CORREAL E, LUTTS S. Seed priming of Trifolium repens L. improved germination and early seedling growth on heavy metal-contaminated soil. Water, Air, & Soil Pollution, 2014, 225(4): 1905-3353.
[25] 张燕, 方力, 吴业池, 冯永新, 姚照兵. PEG浸种处理提高烟草种子活力的效应. 种子, 2003(6): 26-29. doi: 10.3969/j.issn.1000-8071.2003.06.014 ZHANG Y, FANG L, WU Y C, FENG Y X, YAO Z B. Effect of soaking seeds with PEG on improving seed vigor of tobacco. Seed, 2003(6): 26-29. doi: 10.3969/j.issn.1000-8071.2003.06.014
[26] 覃柳燕, 唐美琼, 梁莹, 李林轩, 缪剑华. 不同温度贮藏后山豆根种子酶活性与发芽率相关性研究. 种子, 2011, 30(11): 8-10. doi: 10.3969/j.issn.1000-8071.2011.11.004 QIN L Y, TANG M Q, LIANG Y, LI L X, MIU J H. Study on the relationship between antioxidant enzymes activities and germination rate of Sophora tonkinensis Gagnep seed storaged at different temperatures. Seed, 2011, 30(11): 8-10. doi: 10.3969/j.issn.1000-8071.2011.11.004
[27] REHMAN H U, IQBAL H, BASRA S M A, IRFAN A, MUHAMMAD F, ABDUL W, WANG N. Seed priming improves early seedling vigor, growth and productivity of spring maize. Journal of Integrative Agriculture, 2015, 14(9): 1745-1754. doi: 10.1016/S2095-3119(14)61000-5
[28] LI X M, Zhang L H. SA and PEG-induced priming for water stress tolerance in rice seedling. // Information Technology and Agricultural Engineering. Springer, 2010.
[29] MOHAMMAD A, ABDUL H, SHAO Y H. Seed osmopriming invokes stress memory against post-germinative drought stress in wheat (Triticum aestivum L.). Environmental and Experimental Botany, 2018, 145: 12-20. doi: 10.1016/j.envexpbot.2017.10.002
[30] 王炜, 史雨刚, 王曙光. PEG引发对老化大豆种子发芽及活力的影响. 山西农业科学, 2011, 39(7): 650-654. doi: 10.3969/j.issn.1002-2481.2011.07.07 WANG W, SHI Y G, WANG S G. Effect of PEG on germination and seed vigor of aging soybean. Journal of Shanxi Agricultural Science, 2011, 39(7): 650-654. doi: 10.3969/j.issn.1002-2481.2011.07.07
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