外源水杨酸对草地早熟禾抗褐斑病的诱导与抗病基因PR1和NPR1的表达的影响
English
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参考文献
[1] 董爱香, 胡林, 赵美琦. 草地早熟禾不同品种对褐斑病抗性的差异. 草地学报, 2003, 11(1): 38-41, 57. DONG A X, HU L, ZHAO M Q. Resistance against brown patch of different Poa pratensis cultivar. Acta Agrestia Sinica, 2003, 11(1): 38-41, 57.
[2] 孙建华, 王彦荣, 柴琦. 28个草地早熟禾品种坪用性状评价. 草业科学, 2003, 20(12): 18-21. doi: 10.3969/j.issn.1001-0629.2003.12.005 SUN J H, WANG Y R, CHAI Q. Turf use quality characteristic assessment for 28 varieties of Poa pratensis. Pratacultual Science, 2003, 20(12): 18-21. doi: 10.3969/j.issn.1001-0629.2003.12.005
[3] 高琪昕, 胡新喜, 王欢妍. 水杨酸诱导植物抗病性机制的研究进展. 中国马铃薯, 2014, 28(4): 238-242. doi: 10.3969/j.issn.1672-3635.2014.04.019 GAO Q X, HU X X, WANG H Y. Research progress in mechanism of plant disease resistance induced by salicylic acid. Chinese Potato Journal, 2014, 28(4): 238-242. doi: 10.3969/j.issn.1672-3635.2014.04.019
[4] 孙涛, 曹致中, 马晖玲. 水杨酸诱导苜蓿对霜霉病抗性的研究. 甘肃农业大学学报, 2006, 41(3): 61-64. doi: 10.3969/j.issn.1003-4315.2006.03.014 SUN T, CAO Z Z, MA H L. Study on resistence of alfafa to downy mildew induced by SA. Journal of Gansu Agricultural University, 2006, 41(3): 61-64. doi: 10.3969/j.issn.1003-4315.2006.03.014
[5] 蔡新忠, 郑重, 宋凤鸣. 水杨酸对水稻幼苗抗瘟性的诱导作用. 植物病理学报, 1996, 26(1): 7-12. CAI X Z, ZHENG Z, SONG F M. Effect of salicylic acid on the induction of resistance to rice seedling blast. Acta Phyto Pathologica Sinica, 1996, 26(1): 7-12.
[6] 刘凤权, 王金生. 水杨酸诱导水稻幼苗抗白叶枯病研究. 植物保护学报, 2000, 27(1): 47-52. doi: 10.3321/j.issn:0577-7518.2000.01.010 LIU F Q, WANG J S. Study on the effect of salicylic acid on the resistance of rice seedlings to leaf blight. Journal of Plant Protection, 2000, 27(1): 47-52. doi: 10.3321/j.issn:0577-7518.2000.01.010
[7] 古燕翔, 王代军. 外源诱导物水杨酸对草坪型高羊茅弯孢霉叶病抗性影响的研究. 中国草地, 2003, 25(4): 57-61, 72. GU Y X, WANG D J. Effect of external salicylic acid on tall fescue on Festuca arundinacea Schreb. Glassland of China, 2003, 25(4): 57-61, 72.
[8] 孙艳, 杨淑英. 水杨酸诱发黄瓜幼苗对霜霉病抗性的研究. 植物病理学报, 2004, 34(2): 187-189. doi: 10.3321/j.issn:0412-0914.2004.02.016 SUN Y, YANG S Y. Effects of SA on resistence of cucumber seedlings to downy mildew. Acta Phyto Pathologica Sinica, 2004, 34(2): 187-189. doi: 10.3321/j.issn:0412-0914.2004.02.016
[9] 张莹. 水杨酸诱导玉米抗大斑病的机理研究. 保定: 河北农业大学硕士学位论文, 2007. ZHANG Y. Study on the mechanism of salicylic acid in maize’s resistance to macular disease. Mater Thesis. Baoding: Hebei Agricultural University, 2007.
[10] 金一锋. 外源性水杨酸诱导月季对黑斑病抗性的研究. 哈尔滨: 东北农业大学硕士学位论文, 2013. JIN Y F. Study on resistance of Chinese rose to black spot disease induced of exogenous salicylic acid. Mater Thesis. Harbin: Northeast Agricultural University, 2013.
[11] 房媛媛, 马晖玲. 2,3-丁二醇与水杨酸诱导匍匐翦股颖对镰刀菌枯萎病的抗性. 草原与草坪, 2015, 35(3): 83-87. doi: 10.3969/j.issn.1009-5500.2015.03.017 FANG Y Y, MA H L. 2,3- Butanediol and salicylic acid induced resistance of creeping bentgrass to fusarium wilt. Grassland and Lawn, 2015, 35(3): 83-87. doi: 10.3969/j.issn.1009-5500.2015.03.017
[12] 向妙莲, 汪杰, 阙海勇, 蒋军喜, 罗友强, 刘冰, 宋水林. 水杨酸诱导车前草抗菌核病研究. 江西植保, 2009, 32(3): 111-113, 116. doi: 10.3969/j.issn.1006-4249.2009.03.004 XIANG M L, WANG J, QUE H Y, JIANG J X, LUO Y Q, LIU B, SONG S L. Study on the effect of salicylic acid on the antibacterial nuclear disease of plantain. Jiangxi Plant Protection, 2009, 32(3): 111-113, 116. doi: 10.3969/j.issn.1006-4249.2009.03.004
[13] 钟小刚, 薛应钰, 梁巧兰, 徐秉良, 范祥梅. 水杨酸和壳聚糖诱导苹果叶片对斑点落叶病抗性的研究. 植物保护, 2013, 39(4): 20-24. doi: 10.3969/j.issn.0529-1542.2013.04.005 ZHONG X G, XUE Y Y, LIANG Q L, XU B L, FAN X M. Studies on resistance of salicylic acid and chitosan to leaf spot and defoliate diseaser in apple leaves. Plant Protection, 2013, 39(4): 20-24. doi: 10.3969/j.issn.0529-1542.2013.04.005
[14] BURPEE L, MARTIN B. Biology of rhizoctonia species associated with turgrasses. Plant Disease, 1992, 76(2): 112-117. doi: 10.1094/PD-76-0112
[15] GUO Y H, YU Y P, WANG D. GhZFPl, a novel CCCH-type zinc finger protein from cotton, enhances salt stress tolerance and fungal disease resistance in transgenic tobacco by interacting witn GZIRD21A and GZIPR5. New Phytologist, 2009, 183(4): 62-75.
[16] 殷萍萍. 日本结缕草响应立枯丝核菌侵染及其转录组学研究. 北京: 北京林业大学硕士学位论文, 2015. YIN P P. Study on the transcriptome of zoysiagrass in response to the infection of Rhizoctonia solani. Master Thesis. Beijing: Beijing Forestry University, 2015.
[17] NAKANO Y, ASADAK. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiology, 1981, 22(2): 867-880.
[18] 周丽霞, 易克贤, 马振宇. 苜蓿霜霉病的接种鉴定. 甘肃畜牧兽医, 1998, 140(3): 44. ZHOU L X, YI K X, MA Z Y. Identification of alfafa downy mildew inoculation. Gansu Animalland Veterinary Science, 1998, 140(3): 44.
[19] ALLEN R D. Dissection of oxidative stress tolerance using transgenic plants. Plant Physiology, 1995, 107(4): 1049-1054. doi: 10.1104/pp.107.4.1049
[20] 张岗, 李依民, 张毅. 条锈菌诱导的小麦病程相关蛋白TaPR10基因的克隆及特征分析. 中国农业科学, 2009, 42(1): 110-116. doi: 10.3864/j.issn.0578-1752.2009.01.013 ZHANG G, LI Y M, ZHANG Y. Cloning and characterization of a pathogenesis related protein gene TaPR10 from wheat induced by stripe rust pathogen. Scientia Agricultura Sinica, 2009, 42(1): 110-116. doi: 10.3864/j.issn.0578-1752.2009.01.013
[21] SZALONTAI B, JAKAB G. Differential expression of PRLIPs, a pathogenesis-related gene family encoding class 3 lipase-like proteins in Arabidopsis. Acta Biologica Hungarica, 2010, 61(1): 156-157.
[22] 王坤, 王海燕, 刘大群. 叶锈菌与‘TcLr19’小麦互作体系中PR1基因的克隆及分析. 河北农业大学学报, 2012, 35(2): 1-6. doi: 10.3969/j.issn.1008-6927.2012.02.002 WANG K, WANG H Y, LIU D Q. Cloning and analysis of a PR1 gene from ‘TcLr19’ wheat in the defense responses to Puccinia triticina. Journal of Agricultural University of Hebei, 2012, 35(2): 1-6. doi: 10.3969/j.issn.1008-6927.2012.02.002
[23] CAO H, BOWLING S A, GORDON A S. Characterizaion of an Arabidopsis mutant that is nonresponsive to inducers of systemic acquired resistance. Plant Cell, 1994, 6(8): 1583-1592.
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图 1 接种后午夜草地早熟禾发病率
不同小写字母表示处理A与B间差异显著(P < 0.05)。图2同。
Figure 1. Plant infection percent of Kentucky bluegrass ‘Midnight’ post inoculation
Different lowercase letters indicate significant differences between A and B treatments at the 0.05 level; similarly for the Figure 2.
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图 2 病原菌接种后草地早熟禾的病情指数
Figure 2. The disease index of Kentucky bluegrass ‘Midnight’ post inoculation
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图 3 病菌接种后SA对草地早熟禾午夜褐斑病的诱抗效果
不同小写字母表示不同处理间差异显著 (P < 0.05)。
Figure 3. Efficacy of resistance induced by SA to brown patch in Kentucky bluegrass ‘Midnight’ post pathogen inoculation
Different lowercase letters indicate significant differences between treatments at the 0.05 level.
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图 4 草地早熟禾午夜基因PR1相对表达量
不同小写字母表示相同处理组在不同时间相对表达量的差异显著性(P < 0.05)。T0，SA喷施前；T1，SA喷施后第1天；T2，SA喷施第2天，即病原菌接种前；T3，病菌接种后第3天；T4，病菌接种后第4天；T5，病菌接种后第5天。下同。
Figure 4. The relative expression of PR1 in Kentucky bluegrass ‘Midnight’
Different lowercase letters in the same treatment indicate significant differences among the different times at the 0.05 level; T0, Befor spraying SA; T1, The first day after spraying SA; T2, The second day after spraying SA, before inoculation; T3, The third day after inoculation; T4, The fourth day after inoculation; T5, The fifth day after inoculation; similarly for the following figures.
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图 5 草地早熟禾午夜NPR1基因相对表达量
Figure 5. The relative expression of NPR1 in Kentucky bluegrass ‘Midnight’
表 1 引物序列
Table 1 Primer sequence
引物编号 Primer code 引物序列 Primer sequence (5′-3′) 18S-F GATAGGAAGAGCCGACAT 18S-R ATACGAACCGTGAAAGCG PR1-F CGCTACGCCCGCTCCC PR1-R GCCCCTCGTCCACCCA NPR1-F CAAGGAAGGGCAGACTAA NPR1-R GCAGCGATGTGAAGAACA 
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[1] 董爱香, 胡林, 赵美琦. 草地早熟禾不同品种对褐斑病抗性的差异. 草地学报, 2003, 11(1): 38-41, 57. DONG A X, HU L, ZHAO M Q. Resistance against brown patch of different Poa pratensis cultivar. Acta Agrestia Sinica, 2003, 11(1): 38-41, 57.
[2] 孙建华, 王彦荣, 柴琦. 28个草地早熟禾品种坪用性状评价. 草业科学, 2003, 20(12): 18-21. doi: 10.3969/j.issn.1001-0629.2003.12.005 SUN J H, WANG Y R, CHAI Q. Turf use quality characteristic assessment for 28 varieties of Poa pratensis. Pratacultual Science, 2003, 20(12): 18-21. doi: 10.3969/j.issn.1001-0629.2003.12.005
[3] 高琪昕, 胡新喜, 王欢妍. 水杨酸诱导植物抗病性机制的研究进展. 中国马铃薯, 2014, 28(4): 238-242. doi: 10.3969/j.issn.1672-3635.2014.04.019 GAO Q X, HU X X, WANG H Y. Research progress in mechanism of plant disease resistance induced by salicylic acid. Chinese Potato Journal, 2014, 28(4): 238-242. doi: 10.3969/j.issn.1672-3635.2014.04.019
[4] 孙涛, 曹致中, 马晖玲. 水杨酸诱导苜蓿对霜霉病抗性的研究. 甘肃农业大学学报, 2006, 41(3): 61-64. doi: 10.3969/j.issn.1003-4315.2006.03.014 SUN T, CAO Z Z, MA H L. Study on resistence of alfafa to downy mildew induced by SA. Journal of Gansu Agricultural University, 2006, 41(3): 61-64. doi: 10.3969/j.issn.1003-4315.2006.03.014
[5] 蔡新忠, 郑重, 宋凤鸣. 水杨酸对水稻幼苗抗瘟性的诱导作用. 植物病理学报, 1996, 26(1): 7-12. CAI X Z, ZHENG Z, SONG F M. Effect of salicylic acid on the induction of resistance to rice seedling blast. Acta Phyto Pathologica Sinica, 1996, 26(1): 7-12.
[6] 刘凤权, 王金生. 水杨酸诱导水稻幼苗抗白叶枯病研究. 植物保护学报, 2000, 27(1): 47-52. doi: 10.3321/j.issn:0577-7518.2000.01.010 LIU F Q, WANG J S. Study on the effect of salicylic acid on the resistance of rice seedlings to leaf blight. Journal of Plant Protection, 2000, 27(1): 47-52. doi: 10.3321/j.issn:0577-7518.2000.01.010
[7] 古燕翔, 王代军. 外源诱导物水杨酸对草坪型高羊茅弯孢霉叶病抗性影响的研究. 中国草地, 2003, 25(4): 57-61, 72. GU Y X, WANG D J. Effect of external salicylic acid on tall fescue on Festuca arundinacea Schreb. Glassland of China, 2003, 25(4): 57-61, 72.
[8] 孙艳, 杨淑英. 水杨酸诱发黄瓜幼苗对霜霉病抗性的研究. 植物病理学报, 2004, 34(2): 187-189. doi: 10.3321/j.issn:0412-0914.2004.02.016 SUN Y, YANG S Y. Effects of SA on resistence of cucumber seedlings to downy mildew. Acta Phyto Pathologica Sinica, 2004, 34(2): 187-189. doi: 10.3321/j.issn:0412-0914.2004.02.016
[9] 张莹. 水杨酸诱导玉米抗大斑病的机理研究. 保定: 河北农业大学硕士学位论文, 2007. ZHANG Y. Study on the mechanism of salicylic acid in maize’s resistance to macular disease. Mater Thesis. Baoding: Hebei Agricultural University, 2007.
[10] 金一锋. 外源性水杨酸诱导月季对黑斑病抗性的研究. 哈尔滨: 东北农业大学硕士学位论文, 2013. JIN Y F. Study on resistance of Chinese rose to black spot disease induced of exogenous salicylic acid. Mater Thesis. Harbin: Northeast Agricultural University, 2013.
[11] 房媛媛, 马晖玲. 2,3-丁二醇与水杨酸诱导匍匐翦股颖对镰刀菌枯萎病的抗性. 草原与草坪, 2015, 35(3): 83-87. doi: 10.3969/j.issn.1009-5500.2015.03.017 FANG Y Y, MA H L. 2,3- Butanediol and salicylic acid induced resistance of creeping bentgrass to fusarium wilt. Grassland and Lawn, 2015, 35(3): 83-87. doi: 10.3969/j.issn.1009-5500.2015.03.017
[12] 向妙莲, 汪杰, 阙海勇, 蒋军喜, 罗友强, 刘冰, 宋水林. 水杨酸诱导车前草抗菌核病研究. 江西植保, 2009, 32(3): 111-113, 116. doi: 10.3969/j.issn.1006-4249.2009.03.004 XIANG M L, WANG J, QUE H Y, JIANG J X, LUO Y Q, LIU B, SONG S L. Study on the effect of salicylic acid on the antibacterial nuclear disease of plantain. Jiangxi Plant Protection, 2009, 32(3): 111-113, 116. doi: 10.3969/j.issn.1006-4249.2009.03.004
[13] 钟小刚, 薛应钰, 梁巧兰, 徐秉良, 范祥梅. 水杨酸和壳聚糖诱导苹果叶片对斑点落叶病抗性的研究. 植物保护, 2013, 39(4): 20-24. doi: 10.3969/j.issn.0529-1542.2013.04.005 ZHONG X G, XUE Y Y, LIANG Q L, XU B L, FAN X M. Studies on resistance of salicylic acid and chitosan to leaf spot and defoliate diseaser in apple leaves. Plant Protection, 2013, 39(4): 20-24. doi: 10.3969/j.issn.0529-1542.2013.04.005
[14] BURPEE L, MARTIN B. Biology of rhizoctonia species associated with turgrasses. Plant Disease, 1992, 76(2): 112-117. doi: 10.1094/PD-76-0112
[15] GUO Y H, YU Y P, WANG D. GhZFPl, a novel CCCH-type zinc finger protein from cotton, enhances salt stress tolerance and fungal disease resistance in transgenic tobacco by interacting witn GZIRD21A and GZIPR5. New Phytologist, 2009, 183(4): 62-75.
[16] 殷萍萍. 日本结缕草响应立枯丝核菌侵染及其转录组学研究. 北京: 北京林业大学硕士学位论文, 2015. YIN P P. Study on the transcriptome of zoysiagrass in response to the infection of Rhizoctonia solani. Master Thesis. Beijing: Beijing Forestry University, 2015.
[17] NAKANO Y, ASADAK. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiology, 1981, 22(2): 867-880.
[18] 周丽霞, 易克贤, 马振宇. 苜蓿霜霉病的接种鉴定. 甘肃畜牧兽医, 1998, 140(3): 44. ZHOU L X, YI K X, MA Z Y. Identification of alfafa downy mildew inoculation. Gansu Animalland Veterinary Science, 1998, 140(3): 44.
[19] ALLEN R D. Dissection of oxidative stress tolerance using transgenic plants. Plant Physiology, 1995, 107(4): 1049-1054. doi: 10.1104/pp.107.4.1049
[20] 张岗, 李依民, 张毅. 条锈菌诱导的小麦病程相关蛋白TaPR10基因的克隆及特征分析. 中国农业科学, 2009, 42(1): 110-116. doi: 10.3864/j.issn.0578-1752.2009.01.013 ZHANG G, LI Y M, ZHANG Y. Cloning and characterization of a pathogenesis related protein gene TaPR10 from wheat induced by stripe rust pathogen. Scientia Agricultura Sinica, 2009, 42(1): 110-116. doi: 10.3864/j.issn.0578-1752.2009.01.013
[21] SZALONTAI B, JAKAB G. Differential expression of PRLIPs, a pathogenesis-related gene family encoding class 3 lipase-like proteins in Arabidopsis. Acta Biologica Hungarica, 2010, 61(1): 156-157.
[22] 王坤, 王海燕, 刘大群. 叶锈菌与‘TcLr19’小麦互作体系中PR1基因的克隆及分析. 河北农业大学学报, 2012, 35(2): 1-6. doi: 10.3969/j.issn.1008-6927.2012.02.002 WANG K, WANG H Y, LIU D Q. Cloning and analysis of a PR1 gene from ‘TcLr19’ wheat in the defense responses to Puccinia triticina. Journal of Agricultural University of Hebei, 2012, 35(2): 1-6. doi: 10.3969/j.issn.1008-6927.2012.02.002
[23] CAO H, BOWLING S A, GORDON A S. Characterizaion of an Arabidopsis mutant that is nonresponsive to inducers of systemic acquired resistance. Plant Cell, 1994, 6(8): 1583-1592.
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