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青藏高原垂穗披碱草种质资源遗传多样性的SSR分析

彭语洛, 周青平, 陈仕勇, 陈有军, 李亚萍, 田莉华

彭语洛, 周青平, 陈仕勇, 陈有军, 李亚萍, 田莉华. 青藏高原垂穗披碱草种质资源遗传多样性的SSR分析[J]. 草业科学, 2018, 12(5): 1080-1089. DOI: 10.11829/j.issn.1001-0629.2017-0689
引用本文: 彭语洛, 周青平, 陈仕勇, 陈有军, 李亚萍, 田莉华. 青藏高原垂穗披碱草种质资源遗传多样性的SSR分析[J]. 草业科学, 2018, 12(5): 1080-1089. DOI: 10.11829/j.issn.1001-0629.2017-0689
Yu-luo Peng, Qing-ping Zhou, Shi-yong Chen, You-jun Chen, Ya-ping Li, Li-hua Tian. Genetic diversity of Elymus nutans germplasm resources from the Qinghai-Tibet Plateau in China detected by SSR markers[J]. Pratacultural Science, 2018, 12(5): 1080-1089. DOI: 10.11829/j.issn.1001-0629.2017-0689
Citation: Yu-luo Peng, Qing-ping Zhou, Shi-yong Chen, You-jun Chen, Ya-ping Li, Li-hua Tian. Genetic diversity of Elymus nutans germplasm resources from the Qinghai-Tibet Plateau in China detected by SSR markers[J]. Pratacultural Science, 2018, 12(5): 1080-1089. DOI: 10.11829/j.issn.1001-0629.2017-0689

青藏高原垂穗披碱草种质资源遗传多样性的SSR分析

基金项目: 

西南民族大学研究生创新型项目(CX2017SZ072)

国家重点研发技术(2017YFC0504806)

国家牧草产业技术体系青藏高原牧草育种岗位(CAR34)

四川省教育厅创新团队(14TD0049)

摘要: 采用SSR分子标记技术,对采自青藏高原的30份野生垂穗披碱草(Elymus nutans)种质进行遗传多样性分析。结果表明,1)筛选出的16对引物共获得了116个等位基因位点,92个多态性位点,多态性位点率(PPB)为79.75%,多态性信息(PIC)含量为0.0630.325,平均值为0.188,材料间遗传相似系数(GS)介于0.6920.976,平均值为0.828。2)聚类分析表明,在遗传相似系数0.804处,30份供试材料可聚为四大类,单独聚为一类的09-214表现出与其他材料较远的亲缘关系,主成分分析结果与UPGMA结果基本一致。群体遗传结构分析显示,大部分材料的遗传背景较为单一,群体划分的结果与聚类分析的结果部分保持一致。研究显示,供试种质材料间存在较大的差异,部分材料表现出相对独立的特性,遗传多样性较丰富,可为垂穗披碱草的保护利用、新品种的选育及优良基因的挖掘提供参考依据。

 

English

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  • 收稿日期:  2017-12-19
  • 修回日期:  2018-03-15
  • 发布日期:  2018-05-19

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