纤维素分解菌与乳酸菌复合发酵条件的响应曲面优化分析

张凡凡; 苗芳; 王旭哲; 唐开婷; 马春晖

doi:10.11829/j.issn.1001-0629.2017-0267

纤维素分解菌与乳酸菌复合发酵条件的响应曲面优化分析

石河子大学动物科技学院,新疆石河子 832000

基金项目:

国家自然科学基金项目(31460637)

国家牧草产业技术体系(CARS-34)

新疆研究生科研创新项目(XJGRI2015037)

摘要: 为给今后复合发酵添加剂的开发提供新的途径,研究了纤维素分解菌与乳酸菌复合发酵情况下二者数量均达到最优值的发酵剂量。采用响应曲面设计法,设计乳酸菌(LAB)和真菌的数量为响应值,求出响应值均为最大时的自变量值。自变量为同、异型发酵LAB和纤维素分解菌添加比例及添加剂量,其中同型[植物乳杆菌(Lactobacillus plantarum)+戊糖片球菌(Pediococcus acidilactici)]、异型[布氏乳杆菌(Lactobacillus buchneri)]发酵LAB的添加量均为1×105、3×105和5×105 cfu·g-1(即5、5.48、5.70 lg cfu·g-1),纤维素分解菌的比例为黑曲霉(Aspergillus niger):绿色木霉菌(Trichoderma viride):枯草芽孢杆菌(Bacillus subtilis)=1:1:2、1:2:1和2:1:1,纤维素分解菌的添加剂量为0.1%、0.2%和0.3%。LAB和真菌的数量的测定采用传统菌种计数法计数。结果表明:建立的LAB和真菌数量的二次多项式模型显著性分别为P<0.01和P=0.03,R2分别为0.95和0.74。其中同、异型发酵LAB的添加量及其交互作用对LAB数量的曲面效应影响显著(P<0.05)。真菌的添加量以及同、异型发酵LAB的交互作用对真菌数量的曲面效应影响显著(P<0.05)。最终优化结果为同型发酵LAB添加量为5×105 cfu·g-1(5.70 lg cfu·g-1),异型发酵LAB添加量为4.7×105 cfu·g-1(5.67 lg cfu·g-1),纤维素分解菌比例为2:1:1,添加量为0.3%。

关键词:

English

Condition of fermentation combined with cellulose decomposing and lactic acid bacteria based on the response surface method

College of Animal Science and Technology, Shihezi University, Shihezi 832003, Xinjiang, China

Received Date: 2017-05-22
Publish Date: 2018-03-19

Abstract: The aim of this study was to investigate the fermentation dose at which the optimum fermentation amount can be met with a combination of cellulose decomposing bacteria and lactic acid bacteria, and to provide a new method for the future development of compound fermentation additives. The response surface method was used in the experiment. The number of lactic acid bacteria (LAB) and fungi were calculated as the response values, with which the maximum values of the independent variables were obtained. The independent variables included the doses and proportions of homo- and hetero-fermentative LAB and cellulose decomposing bacteria. Among them, the addition amounts of homo- (Lactobacillus plantarum and Pediococcus acidilactici) and hetero-fermentative (Lactobacillus buchneri) LAB were 1×105, 3×105, and 5×105 cfu·g-1(as 5, 5.48, 5.70 lg cfu·g-1), respectively. The tested ratios of cellulose decomposing bacteria Aspergillus niger:Trichoderma viride:Bacillus subtilis were 1:1:2, 1:2:1, and 2:1:1. The amount of cellulose decomposing bacteria was 0.1%, 0.2%, and 0.3%. The numbers of LAB and fungi were counted using the traditional bacterial count method. The results showed that the significances in the quadratic polynomial models of LAB and fungi were P<0.01 (R2=0.95) and P=0.03 (R2=0.74), respectively. The addition numbers of the homo- and hetero-fermentative LAB and their interactions had a significant effect on quantity of the affected surface of LAB (P<0.05). The addition number of fungi and the interaction of the homo- and hetero-fermentative LAB had a significant effect on the quantity of the affected surface of fungi (P<0.05). The conclusion is that the addition number of homo-fermentative LAB was 5×105 cfu·g-1(as 5.48 lg cfu·g-1), and hetero-fermentative LAB was 4.7×105 cfu·g-1(as 5.67 lg cfu·g-1), the ratio of cellulose decomposing bacteria was 2:1:1, and the addition amount was 0.3%.

Keywords:

microorganism /
homo-fermentative lactic acid bacteria /
hetero-fermentative lactic acid bacteria /
cellulose decomposing bacteria /
fermentation additive /
fermentation conditions /
response surface analysis

HTML全文

参考文献(53)

[1]	王万中. 试验的设计与分析.北京:高等教育出版社,2004.
[1]	Wang W Z.The Design of the Test and Analysis.Beijing:Higher Education Press,2004.(in Chinese)
[2]	Fang J T.Comparison for experimental designs and modeling in response surface methodology.PhD Thesis.Tianjin:Tianjin University,2011.(in Chinese)
[2]	方俊涛. 响应曲面方法中试验设计与模型估计的比较研究.天津:天津大学博士学位论文,2011.
[3]	许晖,孙兰萍,张斌,石亚中.米曲霉固态发酵啤酒糟产α-淀粉酶的优化.农业机械学报,2008,39(1):82-86.
[3]	Xu H,Sun L P,Zhang B,Shi Y Z.Optimization of producing α-amylase from spent brewing grains under solid-state fermentation by Aspergillus oryzae.Transactions of the Chinese Society of Agricultural Machinery,2008,39(1):82-86.(in Chinese)
[4]	Li X H.Forage microbial species and the main role.Feed Industry,2002,23(2):30-32.(in Chinese)
[4]	李晓晖. 饲用微生物的种类和主要作用.饲料工业,2002,23(2):30-32.
[5]	任付平. 复合微生物菌剂在全株玉米青贮中的应用与研究.西安:西北大学硕士学位论文,2007.
[5]	Ren F P.Research and application of compound microbial additives on the whole-crop corn silage.Master Thesis.Xi’an:Northwest University,2007.(in Chinese)
[6]	Zhou L R,Feng L,Cui Z Z,Feng L Z,Feng C.The method of straw degradation with bacterial complex:CN 103194392A.2015.(in Chinese)
[6]	周丽蓉,冯雷,崔泽周,冯琳之,冯采.降解秸秆的微生物复合菌剂及其降解秸秆的方法:CN 103194392A.2015.
[7]	Mihray·Mutallip.Effects on mixed additive of Lactobacillus and cellulose decomposition bacteria on the silage quality.Master Thesis.Urumqi:Xinjiang University,2014.(in Chinese)
[7]	美合热阿依·木台力甫.乳酸菌与纤维素分解菌混合添加剂对青贮饲料品质的影响研究.乌鲁木齐:新疆大学硕士学位论文,2014.
[8]	刘宇,李阳,史同瑞,朱战波.产纤维素酶黑曲霉菌的研究进展.畜牧与兽医,2015,47(6):157-159.
[8]	Liu Y,Li Y,Shi T R,Zhu Z B.The advance of produce cellulose Aspergillus nige.Animal Husbandry and Veterinary Medicine,2015,47(6):157-159.(in Chinese)
[9]	Ge W Z,Li N.The advance of application of Trichoderma viride.Heilongjiang August First Land Reclamation University,2005,17(2):75-80.(in Chinese)
[9]	葛文中,李楠.绿色木霉应用的研究进展.黑龙江八一农垦大学学报,2005,17(2):75-80.
[10]	和小黑,张文举,魏晓燕.枯草芽孢杆菌产酶活性改良研究进展.中国饲料,2014,515(15):8-10.
[10]	He X H,Zhang W J,Wei X Y.The techniques of increasing activity of the enzyme produced by Bacillus subtilis.China Feed,2014,515(15):8-10.(in Chinese)
[11]	Qin Y,Wang W,Guo B J,Wang X C,Zhang W J,Zhou X,Wang X L.Study on Bacillus subtilis strains Q4 and Q10 inhibiting the growth of Escherichia coli and Salmonella in vitro.Chinese Animal Husbandry and Veterinary Medicine,2014,41(1):207-210.(in Chinese)
[11]	秦瑶,王苇,郭秉娇,王熙楚,张文举,周霞,王晓兰.2株枯草芽孢杆菌对大肠杆菌和沙门氏菌的体外抑菌试验研究.中国畜牧兽医,2014,41(1):207-210.
[12]	Lin F C,Li D B.Bacteriolysis of Bacillus subtilis on plant fungal pathogens.Acta Phytopathologica Sinica,2003,33(2):174-177.(in Chinese)
[12]	林福呈,李德葆.枯草芽孢杆菌(Bacilllus subtilis)S9对植物病原真菌的溶菌作用.植物病理学报,2003,33(2):174-177.
[13]	Sheperd A C,Maslanka M,Quinn D,Kung L J.Additives containing bacteria and enzymes for alfalfa silage.Journal of Dairy Science,1995,78(3):565-572.
[13]	Wan L Q,Li X L,He F.The effect of adding lactic acid bacteria and cellulase on alfalfa silage quality.Pratacultural Science,2011,28(7):1379-1383.(in Chinese)
[14]	Han L Y,Yu Z,Zhou H.Effect of lactic acid bacteria inoculants and enzymes on Roegneria turczaninovii silages.Pratacultural Science,2013,30(9):1439-1444.(in Chinese)
[14]	万里强,李向林,何峰.添加乳酸菌和纤维素酶对苜蓿青贮品质的影响.草业科学,2011,28(7):1379-1383.
[15]	韩立英,玉柱,周禾.乳酸菌和纤维素酶对直穗鹅观草青贮的改善效果.草业科学,2013,30(9):1439-1444.
[15]	Zhang S,Lin Z,Yu H C,Luo Z Z,Zhuang Y F.Effect of enzyme and fermented green juice on silage quality of Pennisetum sp. Pratacultural Science,2017,34(8):1755-1761.(in Chinese)
[16]	Cheng T S.Manufacture and Application of Microbial Culture Medium.Beijing:China Agriculture Press,1995.(in Chinese)
[16]	张诗,林芝,余豪闯,罗宗志,庄益芬.酶制剂和绿汁发酵液对巨菌草青贮品质的影响.草业科学,2017,34(8):1755-1761.
[17]	Cho S,Kang J S,Cho K J.Effect of homofermentative and heterofermentative lactic acid bacteria on the quality and aerobic stability of silage:Meta-analysis.Journal of the Korean Society of Grassland and Forage Science,2014,34(4):247-253.
[17]	Gong Y S,Li Y C,Wu W B,Liu S Y.Study on thermophilic cellulose decomposing bacteria screened and mixed fermentation.Jiangsu Agricultural Sciences,2010,20(3):394-396.(in Chinese)
[18]	陈天寿. 微生物培养基的制造与应用.北京:中国农业出版社,1995.
[18]	Xia X C.Study on the strains screening,optimal parameters of detoxification and active products of mixed culture solid fermentation on cotton seed meal.Master Thesis.Nanjing:Nanjing Agricultural University,2009.(in Chinese)
[19]	Hmouni A,Mouria A,Douira A.Biological control of tomato grey fungi with compost water extracts,Trichoderma species and Gliocladium species.Phytopathologia Mediterranea,2006,45(2):110-116.
[19]	Wang X Z.Study on the dynamic variation of mycotoxins during the fermentation and acobic stability of whole plant corn silage.Master Thesis.Shihezi:Shihezi University,2016.(in Chinese)
[20]	宫玉胜,李玉成,吴旺宝,刘珊垚.嗜热纤维素分解菌的筛选及混合发酵研究.江苏农业科学,2010,20(3):394-396.
[20]	Yang J B.Lactic Acid Bacteria:Biological Basis and Application.Beijing:China Light Industry Press,1996.(in Chinese)
[21]	夏新成. 发酵棉籽粕菌种筛选和复合发酵工艺条件及其发酵活性产物研究.南京:南京农业大学硕士学位论文,2009.
[21]	Chen D H,Zhu Y,Gai T X,Zhang B L,Han J H.Inhibition effect of lactic acid bacteria to mold isolated from yogurt.China Dairy,2014,42(1):21-23.(in Chinese)
[22]	Xu C C.Silage Science and Technology.Beijing:Science Press,2013.(in Chinese)
[22]	王旭哲. 全株玉米青贮发酵及有氧稳定期霉菌毒素动态变化研究.石河子:石河子大学硕士学位论文,2016.
[23]	Fu C L.Study on regularity of mildew in the silage and the screening of mould inhibitor.Master Thesis.Zhengzhou:He’nan Agricultural University,2012.(in Chinese)
[23]	杨洁彬.乳酸菌:生物学基础及应用.北京:中国轻工业出版社,1996.
[24]	Gourama H,Bullerman L B.Antimycotic and antiaflatoxigenic effect of lactic acid bacteria:A review.Journal of Food Protection,1995,58(11):1275-1280.
[25]	陈大欢,朱忆,盖天欣,张柏林,韩俊华.乳酸菌对酸乳中霉菌的抑制作用研究.中国乳品工业,2014,42(1):21-23.
[26]	徐春城. 现代青贮理论与技术.北京:科学出版社,2013.
[27]	付春丽. 青贮饲料霉变规律及防霉剂筛选研究.郑州:河南农业大学硕士学位论文,2012.
[28]	Gerez C L,Torres M J,Valdez G F D,Rollán G.Control of spoilage fungi by lactic acid bacteria.Biological Control,2013,64(3):231-237.
[29]	Filya I,Muck R E,Contreras-Govea F E.Inoculant effects on alfalfa silage:Fermentation products and nutritive value.Journal of Dairy Science,2007,90(11):5108-14.
[30]	Lindseyj R,Limin K.Effects of combining Lactobacillus buchneri 40788 with various lactic acid bacteria on the fermentation and aerobic stability of corn silage.Animal Feed Science and Technology,2010,159(3-4):105-109.

资源附件(0)

计量

PDF下载量: 138
文章访问数: 1276
HTML全文浏览量: 179
被引次数: 0

纤维素分解菌与乳酸菌复合发酵条件的响应曲面优化分析

English

Condition of fermentation combined with cellulose decomposing and lactic acid bacteria based on the response surface method

计量

文章相关

目录