不同基因型狗牙根耐镉及镉富集特性

刘翔宇; 李晓宁; 韩世娟; 谢燕

doi:10.11829/j.issn.1001-0629.2016-0399

不同基因型狗牙根耐镉及镉富集特性

1.
山东省东营市第一中学,山东东营 257091
2.
中国科学院武汉植物园植物种植资源创新与特色农业重点实验室,湖北武汉 430074

基金项目:

国家自然青年科学基金(31502009)

摘要: 狗牙根(Cynodon dactylon)作为我国南方重金属污染区的优势种,在污染土壤的修复过程中有巨大的应用潜力。本研究利用水培试验,分析了10个狗牙根基因型生长指标的变化、耐镉能力及镉吸收转运特征。结果表明,1.5 mmol·L-1镉胁迫条件下,狗牙根各基因型均表现出生长缓慢、叶片失绿、根系生长不良等受害症状。不同基因型的受害程度差异较大,其中,基因型WBD242、WBD245和WBD193的受害程度较轻。综合评价结果表明,这3个基因型对镉具有很强的耐受性,且其地上部积累的镉离子浓度分别高达119.0、177.0和124.2 mg·kg-1,均高于超富集植物的标准(地上部分富集镉超过100 mg·kg-1),可用于高浓度镉污染土壤的植物修复;基因型WBD144和WBD147具有最高的转移系数,但是耐镉能力较弱,可用于轻度镉污染土壤的植物修复。研究结果为镉污染土壤的植物修复与植被重建提供了新的材料和理论基础。

关键词:

English

Cadmium tolerance and accumulation in different Bermuda grass genotypes

1.
The First Secondary School, Dongying 257091, China
2.
Key Laboratory of Plant Germplasm Innovation and Characteristic Agriculture, Wuhan Plant Garden, Chinese Academy of Sciences, Wuhan 430074, China

Received Date: 2016-07-24
Publish Date: 2016-12-19

Abstract: As a common and dominant species in heavy metal contaminated soils in southern China, Bermuda grass can be potentially used for Cd phytoremediation. The growth response following exposure and tolerance to Cd, as well as accumulation of Cd of 10 Bermuda grass genotypes were studied under hydroponic culture conditions with 1.5 mmol·L-1 of Cd. The results indicated that under Cd stress conditions, all of the Bermuda grass genotypes appeared to show symptoms such as slow growth, drawl plant, yellow and wilting leaves, and poor root growth. Differences in the degree of damage in different genotypes were significant. Among them, three genotypes (WBD242, WBD245, and WBD193) exhibited less severe damage in turf quality, chlorophyll content, and transpiration rate under Cd stress. These three genotypes showed the strongest Cd accumulation ability (119.0, 177.0, 124.2 mg·kg-1 in aboveground parts, respectively), which surpassed the putative criteria for hyper-accumulation plants expected to be used for phytoremediation of severe Cd contaminated soil. However, we also found that two other accessions, WBD144 and WBD147, showed the highest translocation capacity for Cd, but with weaker Cd tolerance, which could be applied for phytoremediation of slightly polluted area. Our findings could be used as guidance for future studies on phytoremediation and reconstruction.

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不同基因型狗牙根耐镉及镉富集特性

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Cadmium tolerance and accumulation in different Bermuda grass genotypes

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