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亚精胺对PEG渗透胁迫下白三叶种子萌发及幼苗抗旱效应的影响

彭丹丹, 王晓娟, 李 州, 李亚萍, 彭 燕

彭丹丹, 王晓娟, 李 州, 李亚萍, 彭 燕. 亚精胺对PEG渗透胁迫下白三叶种子萌发及幼苗抗旱效应的影响[J]. 草业科学, 2016, 10(9): 1739-1746. DOI: 10.11829/j.issn.1001-0629.2015-0657
引用本文: 彭丹丹, 王晓娟, 李 州, 李亚萍, 彭 燕. 亚精胺对PEG渗透胁迫下白三叶种子萌发及幼苗抗旱效应的影响[J]. 草业科学, 2016, 10(9): 1739-1746. DOI: 10.11829/j.issn.1001-0629.2015-0657
Dan-dan Peng, Xiao-juan Wang, Zhou Li, Ya-ping Li, Yan Peng. Effects of spermidine on seed germination and seedling drought resistance of white clover under osmotic stress induced by PEG[J]. Pratacultural Science, 2016, 10(9): 1739-1746. DOI: 10.11829/j.issn.1001-0629.2015-0657
Citation: Dan-dan Peng, Xiao-juan Wang, Zhou Li, Ya-ping Li, Yan Peng. Effects of spermidine on seed germination and seedling drought resistance of white clover under osmotic stress induced by PEG[J]. Pratacultural Science, 2016, 10(9): 1739-1746. DOI: 10.11829/j.issn.1001-0629.2015-0657

亚精胺对PEG渗透胁迫下白三叶种子萌发及幼苗抗旱效应的影响

基金项目: 

国家自然科学基金(31372371)

四川省科技支撑项目(2013NZ0013)

摘要: 以‘拉丁诺’白三叶(Trifolium repens cv. Ladino)为试材,研究不同浓度PEG-6000(聚乙二醇-6000)渗透胁迫下亚精胺(spermidine,Spd)对白三叶种子萌发和幼苗抗旱的影响。结果表明,20 μmol·L-1 Spd浸种能够显著(P0.05)提高10%和15% PEG-6000渗透胁迫下白三叶种子的萌发率和萌发指数,显著促进15% PEG-6000渗透胁迫下胚根的伸长生长和幼苗的单株鲜重,但对单株干重影响很小;20% PEG-6000渗透胁迫下,外源添加20 μmol·L-1 Spd能不同程度地提高白三叶幼苗叶片超氧化物歧化酶(superoxide dismutase,SOD)、过氧化物酶(peroxidase,POD)、过氧化氢酶(catalase,CAT)和抗坏血酸过氧化物酶(ascorbate peroxidase,APX)活性,降低电解质渗透率(EL)和丙二醛(MDA)含量,有效缓解叶片相对含水量(RWC)下降。表明渗透胁迫下Spd能有效促进白三叶种子的萌发,增强幼苗抗氧化能力,从而减轻渗透胁迫对白三叶幼苗的过氧化伤害,提高植株的耐旱性。

 

English

  • [1]

    Dong K H,Shen Y X.Forage Production.Beijing:Chinese Agriculture Press,1995:78-79.(in Chinese)

    [1] 董宽虎,沈益新.饲草生产学.北京:中国农业出版社,1995:78-79.
    [2] 李志亮,邢浩春,杨清,吴忠义,张秀海,黄丛林.白三叶转基因研究进展.北方园艺,2009,3(7):149-152.
    [2]

    Li Z L,Xing H C,Yang Q,Wu Z Y,Zhang X H,Huang C L.Advancement of the research on transgenic white clover.Northen Horticulture,2009,3(7):149-152.(in Chinese)

    [3]

    Kusano T,Yamaguchi K,Berberich T,Takahashi Y.Advances in polyamine research in 2007.Journal of Plant Research,2007,120:345-50.

    [3]

    Zhang C M,Zou Z R,Zhang Z X.Effects of exogenous spermidine on reactive oxygen levels and antioxidative system of tomato seedling under polyethlene glycol stress.Chinese Journal of Applied Environment Biology,2009,15(3):301-307.(in Chinese)

    [4]

    Zou H.Mechanism research on drought tolerance of Agrostis stolonifera Induced by pretreatment of spermidine.Master Thesis.Chengdu:Sichuan Agricultural University,2012.(in Chinese)

    [4]

    Sarvajeet S G,Narendra T.Polyamines and abiotic stress tolerance in plants.Plant Signaling Behavior,2010,5(1):26-33.

    [5]

    Rubén A,Teresa A,Francisco M,Cristina B,Matthieu R,Csaba K,Pedro C,Antonio F T.Polyamines:Molecules with regulatory functions in plant abiotic stress tolerance.Planta,2010,231:1237-1249.

    [5]

    Xia F S,Mao P S,Yan H F,Wang M Y,Zheng H M,Chen Q Z.Effects of salicylic acid on stress resistance of seeds and seedling.Patacultural Science,2014,31(7):1367-1373.(in Chinese)

    [6]

    Zhao B,Yang X Y,Shen X Q.Effects of drought and cold stress on seeds germination of Thymus vulgaris.Patacultural Science,2015,32(11):1808-1814.(in Chinese)

    [6]

    Galston A W,Sawhney R K,Altabella T.Chilling-stress responses by rice seedlings growth with different ammonium concentrations and its relationship to leaf spermidine content.Journal of Plant Biology,2012,22:191-197.

    [7]

    Hu X H,Zou Z R,Yang Z C.Effects of exogenous spermidine on the germination of tomato seeds under salt stress.Northen Horticulture,2009,11(7):1-3.(in Chinese)

    [7]

    Yamamoto A,Shim I S,Fujihara S.Chilling-stress responses by rice seedlings grown with different ammonium concentrations and its relationship to leaf spermidine content.Journal of Plant Biology,2012,55:191-197.

    [8]

    Zhang S Y,Ren X L,Cheng S C,Li S J.Effects of seed soaking with exogenous nitric oxide on the seed germination and seedling growth of maize.Plant Physiology Communications,2004,40(3):309-310.(in Chinese)

    [8]

    Farooq M,Basra S M A,Hussain M,Rehman H,Saleem B A.Incorporation of polyamines in the priming media enhances the germination and early seedling growth in hybrid sunflower (Helianthus annuus L.).International Journal of Agriculture and Biology,2007,9:868-872.

    [9]

    Bai X,Su G X,Sun N,Wang L W,Gu F G.Relationship between exogenous polyamines and nitric oxide and their induced effects on lettuce seed germination.Acta Botanica Boreali-Occidentalia Sinica,2009,29(9):1860-1866.(in Chinese)

    [9]

    Cao D D,Hu J,Zhu S J.Relationship between changes in endogenous polyamines and seed quality during development of sh 2 sweet corn (Zea mays L.) seed.Scientia Horticulturae,2010,123(3):301-307.

    [10]

    Shi L G,Zhou L,Liu H P.Effects of seed soaking with exogenous spermidine on tolerance of wheat (Triticum aestivum L.) seedling to osmotic stress.Journal of Henan Agricultural Science,2008,7:29-32.(in Chinese)

    [10]

    Kubi J.Exogenous spermidine differentially alters activities of some scavenging system enzymes,H2O2 and superoxide radical levels in water-stressed cucumber leaves.Journal of Plant Physiology,2008,165:397-406.

    [11]

    Bo S H.Effects of exogenous spermidine on senescence of detached soybean leaves.Chinese Agricultural Science Bulletin,2008,24(6):243-246.(in Chinese)

    [11]

    Tian J,Wang L P,Yang Y J,Sun J,Guo S R.Exogenous spermidine alleviates the oxidative damage in cucumber seedlings subjected to high temperatures.Jornal of the American Society for Horticultural Science,2012,137:11-19.

    [12]

    Jin Z M,Sha W,Zang W,Sun W,Chai H.Effects of drought stress on protective enzymes of Trifolium repens seedlings.Journal of Northeast Forestry University,2010,38(7):52-53.(in Chinese)

    [12]

    He L,Ban Y,Inoue H,Matsuda N,Liu J,Moriguchi T.Enhancement of spermidine content and antioxidant capacity in transgenic pear shoots overexpressing apple spermidine synthase in response to salinity and hyperosmosis.Phytochemistry,2008,69:2133-2141.

    [13]

    Sun N.The Interaction between polyamines and no signalling molecule in the process of root growth and development in lettuce seedlings.Master Thesis.Suzhou:Soochow University,2011.(in Chinese)

    [13]

    Parvin S,Lee O R,Sathiyaraj G,Khorolragchaa A,Kim Y J,Yang D C.Spermidine alleviates the growth of saline-stressed ginseng seedlings through antioxidative defense system.Gene,2014,537:70-78.

    [14]

    He M,Xu P F,Zhao B C,Li H X,Li Q,Zhang Y N.Physiological responses of Miscanthus sinensis seedlings to PEG water stress.Patacultural Science,2014,31(2):243-249.(in Chinese)

    [14] 张春梅,邹志荣,张志新.外源亚精胺对模拟干旱胁迫下番茄幼苗活性氧水平和抗氧化系统的影响.应用与环境生物学报,2009,15(3):301-307.
    [15] 邹慧.亚精胺预处理诱导匍匐剪股颖抗旱性的生理机制研究.成都:四川农业大学硕士学位论文,2012,06.
    [15]

    Zhang S,Liu H P,Chen L,Liu Y L.Spermidine enhanced resistance of soybean seedling under water stress.Acta Agriculturae Boreali-Sinica,2005,20(4):25-27.(in Chinese)

    [16] 夏方山,毛培胜,闫慧芳,王明亚,郑慧敏,陈泉竹.水杨酸对植物种子及幼苗抗逆性的影响.草业科学,2014,31(7):1367-1373.
    [16]

    Duan H G,Lei T,Qing D H,Xi D H,Sun X,Shan J,Lin H H.The Effects of spermidine physiological activities of wheat seedlings under osmotic stress.Journal of Sichuan University:Natural Science Edition,2006,43(4):922-926.(in Chinese)

    [17] 赵波,杨秀云,沈向群.PEG与低温对法国百里香种子萌发的影响.草业科学,2015,32(11):1808-1814.
    [17]

    Xu Q H,Dong S T,Gao R Q.The effects of exogenous glucose,fructose and no donor sodium nitroprusside(SNP) on maize seed germination.Journal of Maize Science,2008,16(3):309-310.(in Chinese)

    [18]

    Zhou L,Hui Z,Yan P,Xin Q Z,Xiao M,Lin K H,Yan H Y.Exogenously applied spermidine improves drought tolerance in creeping bentgrass associated with changes in antioxidant defense,endogenous polyamines and phytohormones.Plant Growth Regulation,2015,76(1):71-82.

    [19] 中华人民共和国农业部农药室内生物测定试验准则 植物生长调节剂 第1部分:促进/抑制种子萌发试验 浸种法:中国,NY/T 2061.1-2011.
    [20]

    Zhang S,Hu J,Zhang Y,Xie X J,Knapp A.Seed priming with brassinolide improves lucerne (Medicago sativa L.) seed germination and seedling growth in relation to physiological changes under salinity stress.Australian Journal of Agricultural Research,2007,58:811-815.

    [21] 高俊凤.植物生理学实验指导.北京:高等教育出版社,2006:144-148.
    [22] 李合生.植物生理生化实验原理和技术.北京:高等教育出版社,2000:169-172.
    [23]

    Giannopolitis C N,Ries S K.Superoxide dismutase I.Occurrence in higher plants.Plant Physiology,1977,59:309-314.

    [24]

    Chance B,Maehly A C.Assay of catalase and peroxidase.Methods in Enzymology,1955,2:764-775.

    [25]

    Nakano Y,Asada K.Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts.Plant and Cell Physiology,1981,22:867-880.

    [26]

    Bradford M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Analytical Biochemistry,1976,72:248-254.

    [27]

    Dhindsa R S,Dhindsa P,Thorpe T A.Leaf senescence:Correlated with increased leaves of membrane permeability and lipid peroxidation,and decreased levels of superoxide dismutase and catalase.Journal of Experiment Botany,1981,32:93-101.

    [28]

    Barrs H D,Weatherley P E.A re-examination of the relative turgidity technique for estimating water deficits in leaves.Australian Journal of Biological Science,1962,15:413-428.

    [29]

    Blum A,Ebercon A.Cell membrane stability as a measure of drought and heat tolerance in wheat.Crop Science,1981,21:43-47.

    [30]

    Delachiave M E A,Pinho S Z.Germination of Senna occidentalis link:Seed at different osmotic potential levels.Brazilian Archives of Biology and Technology,2003,46(2):163-166.

    [31] 胡晓辉,邹志荣,杨振超.亚精胺对盐胁迫下番茄种子萌发的影响.北方园艺,2009,11(7):1-3.
    [32] 张少颖,任小林,程顺昌,李善菊.外源一氧化氮供体浸种对玉米种子萌发和幼苗生长的影响.植物生理学通讯,2004,40(3):309-310.
    [33] 白旭,苏国兴,孙娜,王立伟,顾福根.外源多胺对莴苣种子萌发诱导及其与一氧化氮的关系.西北植物学报,2009,29(9):1860-1866.
    [34] 史留功,周琳,刘怀攀.亚精胺浸种对小麦幼苗抗渗透胁迫能力的影响.河南农业科学,2008,7:29-32.
    [35] 柏素花.外源亚精胺对大豆离体叶片衰老的影响.中国农学通报,2008,24(6):243-246.
    [36] 金忠民,沙伟,臧威,孙微,柴华.干旱胁迫对白三叶幼苗保护酶的影响.东北林业大学学报,2010,38(7):52-53.
    [37] 孙娜.多胺和一氧化氮信号分子在莴苣幼苗根系发育中的相互作用.苏州:苏州大学硕士学位论文,2011.
    [38] 何淼,徐鹏飞,赵保成,李海芯,李强,张彦妮.中国芒幼苗对 PEG 胁迫的生理响应.草业科学,2014,31(2):243-249.
    [39] 张胜,刘怀攀,陈龙,刘友良.亚精胺提高大豆幼苗的抗旱性.华北农学报,2005,20(4):25-27.
    [40] 段辉国,雷韬,卿东红,席德慧,孙歆,单娟,林宏辉.亚精胺对渗透胁迫小麦幼苗生理活性的影响.四川大学学报:自然科学版,2006,43(4):922-926.
    [41] 徐勤虎,董树亭,高荣岐.外源果糖、葡萄糖与一氧化氮供体(SNP)对玉米种子萌发的影响.玉米科学,2008,16(3):309-310.
    [42]

    Mostofa M G,Yoshida N,Fujita M.Spermidine pretreatment enhances heat tolerance in rice seedlings through modulating antioxidative and glyoxalase systems.Plant Growth Regulation,2014,73:31-44.

    [43]

    Farooq M,Wahid A,Lee D J.Exogenously applied polyamines increase drought tolerance of rice by improving leaf water status,photosynthesis and membrane properties.Acta Physiologiae Plantarum,2009,31:937-945.

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  • 收稿日期:  2015-11-22
  • 发布日期:  2016-09-19

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