两种化学诱变剂对沙芦草染色体的加倍效果

周璐璐; 伏兵哲; 许冬梅; 高雪芹

doi:10.11829/j.issn.1001-0629.2015-0519

两种化学诱变剂对沙芦草染色体的加倍效果

周璐璐^1,2,3,
伏兵哲^1,2,3,
许冬梅^1,2,3,
高雪芹⁴

1.
1. 宁夏大学农学院草业科学研究所,宁夏银川 750021
2.
2. 宁夏大学西北土地退化与生态恢复省部共建国家重点实验室培训基地,宁夏银川 750021
3.
4.
宁夏大学科学技术处,宁夏银川 750021

基金项目:

宁夏高等学校科学研究项目(NGY2013026)

宁夏自然科学基金(NZ2013030)

宁夏牧草育种专项子课题(2014NYYZ040101)

摘要: 为提高沙芦草(Agropyron mongolicum)的牧草品质,筛选最优的染色体加倍方案,采用不同浓度的秋水仙素(0.02%,0.05%,0.08%,0.11%,0.14%+1.5%二甲基亚枫)和氟乐灵(1,5,10,15,20 μmol·L-1)处理沙芦草种子,并进行表观形态及染色体数目的鉴定。结果表明,经处理的沙芦草种子成活后的变异植株均为二倍体与四倍体的混倍体;0.11%秋水仙素+1.5%二甲基亚砜处理20 h的染色体数目变异率最大,高达50%,其成活率为40%;1 μmol·L-1氟乐灵处理6 h变异效果显著,变异率达38.46%。本研究为后期进一步筛选沙芦草加倍植株奠定了理论与试验基础。

关键词:

English

Effects of chemical mutagens on chromosome doubling of Agropyron mongolicum

Lu-lu Zhou^1,2,3,
Bing-zhe Fu^1,2,3,
Dong-mei Xu^1,2,3,
Xue-qin Gao⁴

1.
1. Grassland Science Institute, Agricultural College, Ningxia University, Yinchuan 750021, China
2.
2. Breeding Base for State Key Labotatory of Land Degradation and Ecological Restoration in Northwest China, Ningxia University, Yinchuan 750021, China
3.
4.
Science and Technology Department, Ningxia University, Yinchuan 750021, China

Received Date: 2015-09-15
Publish Date: 2016-05-19

Abstract: In order to improve the forage quality of Agropyron mongolicum and screen the best chromosome doubling treatment, the seeds of A. mongolicum were treated with different concentrations of Colchicine (0.02%,0.05%,0.08%,0.11%,0.14%+1.5% DMSO) and Trifluralin (1,5,10,15,20 μmol·L-1) and the morphology and chromosome number were identified after treatment. The results showed that the surviving plants after treated were mixoploid of diploid and tetraploid. The treatment with 0.11% colchicine 1.5% DMSO for 20 h had the highest mutation rate of 50% and the survival rate of 40%. The treatment with 1 μmol·L-1 trifluralin for 6 h had significant mutation effect with probability of 38.46%. These results provide theoretical and experimental basis for further screening the chromosome doubling plants of A. mongolicum.

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两种化学诱变剂对沙芦草染色体的加倍效果

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Effects of chemical mutagens on chromosome doubling of Agropyron mongolicum

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