application on isoflavonoides content of Minshan red clover

The influence of rhizobia dose, inoculation methods and nitrogen fertilizer dose on the content of isoflavonoides in Trifolium pretense cv. Mingshan was study in an orthogonal experiment with three factors (34). The results showed that inoculation rhizobia affected the total content and output of per unit area of isoflavonoides significantly (P＜0.05), in which 16g rhizobia per kilogram seeds had the best effect. Seed treatment and nonitrogen fertilizer treatment were better than others, thought no significant differences were found between inoculation methods and nitrogen fertilizer tests. In this study, inoculating rhizobia in 2 cm soil depth without fertilizer N seems to be helpful for increasing the production of isoflavonoides from the red clover.