Effect of low energy N╋ beams on germination ability of Cynodon dactylon seeds

Study on germination of M1 generation seeds from Cynodon dactylon was to establish feasible implantation parameters and to understand mutation effect of N+ beams on C. dactylon and its mechanism. Naturally dry seeds were treated through N+ beams from LZD1000 machine, and the change on germination ability of seeds aroused from N+ beams was studied. The results indicated that the appropriate dose of N+ beams was able to stimulate seeds germinating if the implantation time was in 5 seconds, the germination ability would decrease if over 5 seconds. Three values of germination rate, germination energy and vigor index of seeds through six dose treatments with 5 seconds were more than that in control by 10%,9% and 16. But three values of controlled seeds with 10 s treatment time were lower than control by 55%, 15% and 134. There were significantly differences in effect of different doses on germination rate, germination energy and vigor index ( the F values of three indexes were 638.13,109.31,2.16, and higher than F0.05（12, 26）=2.15). It is concluded that 5s implantation time and 801014 ions/cm2 dosage were best mutation parameters under 20 kev energy, and this could induce more variation on seed germination of C.dynodon.