Identification of stress resistant transgenic ryegrass and tall fescue plants expressing ABP9 gene

Plant expression vectors on which the maize bZIP transcriptional factor ABP9 was under the control of either a constitutive promoter Ubi/35S or an ABA responsive promoter were constructed and transformed into ryegrass and tall fescue via Agrobacterium mediated transformation or particle bombardiment, and the resulting regenerated plants were analyzed by PCR, Southern and Northern blotting for integration and expression of ABP9 and tested for resistance under drought or high salinity conditions. The results showed that transgenic ryegrass and tall fescue plants expressing ABP9 with enhanced stress tolerance have been obtained.