Screening and identification of yeast for tolerance to high temperature, alcohol and low acid

Yeast from the different samples such as the polluted water, soil and slightly rot fruit and so on was enriched, separated, purified and selected, and 4 yeast strains for tolerance to high temperatures, low acid and alcohol, named JM-1,JM-2,JM-3 and JM-4, were obtained. The results showed that the best suitable to five-carbon sugar concentration, alcohol, acidity and temperature of 4 yeast strains were 12 g·L-1, 14% (volume ratio), between 2.5 to 3.0 as well as 37 ℃ by morphological and physiological characterization respectively. The results indicated that strains JM-1, JM-2 and JM-4 belonged to the genus Meyerozyma, and strain JM-3 belonged Pichia using sequence analysis of 26S rDNA D1/D2 region. Selected yeast strains expressed good quality and performance on behalf of tolerance to high temperature, alcohol, low acid, and had some research significance.