SPAD value of different parts in 5 forage grass leaves

In the present study, SPAD values of different positions on the same leaf were measured using the equipment SPAD-502(Soil Plant Analysis Development) for 5 herbage grasses, including Avena sativa cv. Qinghai No.1, A.sativa cv. Qinghai, Secale cereal, Lolium multiflorum cv. Tetragold, and L.multiflorum cv. Changjiang No.2. The results indicated that SPAD values of A.sativa cv. Qinghai No.1 and A. sativa cv. Qinghai were significantly higher than that of S.cereal,L. multiflorum cv. Tetragold and L. multiflorum cv. Changjiang No.2. Moreover, the SPAD value stabilization varied from position to position on the same leaf. SPAD value from the same leaf declined from the tip to the middle part and to the bottom of a leaf. The measured SPAD values at the middle part of a leaf were more stable than those from the tip and the bottom which could be used as the appropriate candidate part for SPAD measure.