The clone of TrMYB1R1 from white clover and gene expression analysis

R1-MYB, a subtribe of the MYB family, may be involved in responses to abiotic stress in plants. In this study, a MYB transcription factor ‘TrMYB1R1’ was cloned from white clover (Trifolium repens) through RT-PCR and RACE. The complete sequence of TrMYB1R1 is 1 403 bp encoding 297 amino acids. Based on the analysis of information biology, TrMYB1R1 contains a MYB structural domain and its conservative domain could form 3 alpha-helixes, indicating that TrMYB1R1 is a MYB-related protein. TrMYB1R1 was located in the nucleus through prediction of the subcellular localization. The result of homologous evolution analysis showed that TrMYB1R1 has closer genetic relationships with MYB of Leguminosae. The TrMYB1R1 could be induced in leaves and roots of white clover under abiotic stress and phytohormone treatments. In detail, the expression of TrMYB1R1 was inhibited under water stress (PEG), cadmium, cold (4 °C), and heat (35 °C) stress. More importantly, cadmium and cold had stronger effects on the expression of TrMYB1R1 than PEG treatment. Differently, salt (NaCl) stress could significantly induce the increase in the expression of TrMYB1R1. For the phytohormone treatments, abscisic acid (ABA) inhibited the expression of TrMYB1R1, whereas cytomin (CTK) induced its expression. All of these results indicated that TrMYB1R1 could be involved in responses to abiotic stress and phytohormones, but the function of TrMYB1R1 still requires further investigation in white clover.