Isolation of Lactobacillus producing conjugated linoleie acid in Qinghai area and its effect on quality of whole corn silage

A bacteria which could convert linoleic acid to conjugated linoleie acid (CLA), was screened from the silage in Qinghai Province. The bacteria was identified by morphological observation and 16S rDNA gene sequence analysis. The bacteria was inoculated to the whole plant corn and was cultured 40 days in seal silage bags. The effects of bacteria on the organoleptic properties and chemical compositions of the whole corn silages were assessed. The results showed that this bacteria strain producing CLA was Lactobacillus plantarum and named ANCLA2. It can effectively improve the content of lactic acid and acetic acid in silage, reduce pH and inhibit the ammonia nitrogen (NH₃-N) content, but the dry matter (DM) content of whole plant corn and the crude protein (CP) significantly decreased, and the more the dry matter was lost with the inoculation increasing; neutral detergent fiber (NDF) and acid detergent fiber (ADF) were significantly higher than the control group (P < 0.05), crude fat (EE) and CLA content higher than other treatment groups (P < 0.05). In summary, ANCLA2 bacteria not only ensure the effect of the whole plant corn silage, but also improve the content of crude fat and CLA.