Symptoms and pathogen detection of alfalfa virus disease

In order to investigate the symptoms, pathogen and host range of alfalfa virus diseases in Lanzhou City, Gansu Province, alfalfa virus diseases were investigated in field and pathogens were tested by DAS-ELISA. The results showed that the viral disease of alfalfa widely spread in Anning District and Gaolan County of Lanzhou and Jingtai County of Baiyin City and the highest incidence and disease index were 43.33% and 26.08, respectively, which were in Jingtai. A total of 90 sample were collected and 30 of them were detected which brought the detection ratio of 30%. Alfalfa mosaic virus (AMV), white clover mosaic virus(WCMV), bean yellow mosaic virus (BYMV) and cowpea mosaic virus (CPMV) were main viral pathogen which had detection rates of 100.00%, 50.00%, 75.00%, 75.00%, respectively, and their mixed infection rate of 50.00%. There were 6 families, 17 genera and 18 species for host range testing. AMV can infect 6 families, 15 species, WCMV can infect 4 families, 8 species, BYMV and CPMV could infect 3 families, 7 species, and four kinds of virus could co-infect 6 families, 14 species of plants.