Establishment of the rapid propagation system from stem and leaf explant of Chrysanthemum×grandiflora ‘niu9717’

In vitro micropropagation and shoot regeneration were studied using axillary bud and leaves of Chrysanthemum×grandiflora ‘niu9717’ aseptic stems as explants. The optimal proliferation medium was MS MS+6-BA 0.3 mg·L-1+KT 0.5 mg·L-1+NAA 0.5 mg·L-1 which had average multiplication coefficient of 3.2. The optimal medium for adventitious bud induction from leaves was MS+6-BA 2.0 mg·L-1+NAA 0.5 mg·L-1 which had the callus induction ratio of 100％、adventitious bud differentiation ratio of 92.3％and the reproduction coefficient of 8.9. The combination of 6-BA and NAA was better than that of 6-BA and 2, 4-D or others. Cytokinin KT and TDZ were not conducive to callus induction and adventitious bud differentiation. Auxin 2, 4-D were conducive to callus induction but not to adventitious bud differentiation. The medium of 1/2MS+IBA 0.05 mg·L-1 was the optimal for rooting, which had rooting rate of 100％ and the average root number of 12.08 and average root length of 1.5 cm. The plants after transplanting grew very well with a survival rate of 100％. The high concentration of auxin could induce root but inhibit elongation.